Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
First Claim
1. A method of sequencing a DNA molecule, said method comprising:
- enzymatically producing four sets of primer extension products using said DNA molecule as a template, wherein each of said sets comprises a family of different sized primer extension products terminating at the same base, and further wherein each of said sets comprises the same fluorescent label, wherein the fluorescent labels from at least two of said sets comprise the same donor and acceptor fluorophores in energy transfer relationship separated by different distances and capable of providing distinguishable fluorescence emission patterns, wherein all of the labels of said set are acceptor fluorophores selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes;
size separating said primer extension products;
detecting said size separated primer extension products; and
determining the sequence of said DNA molecule from said detected size separated primer extension products.
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Abstract
Sets of fluorescent energy transfer labels and methods for their use in multi component analysis, particularly nucleic acid enzymatic sequencing, are provided. In the subject sets, at least two of the labels are energy transfer labels comprising a common donor and acceptor fluorophore in energy transfer relationship separated by different distances and capable of providing distinguishable fluorescence emission patterns upon excitation at a common wavelength. The subject labels find particular use in a variety of multi-component analysis applications, such as probes in FISH and multi array analyses, as well as primers in nucleic acid enzymatic sequencing applications.
150 Citations
10 Claims
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1. A method of sequencing a DNA molecule, said method comprising:
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enzymatically producing four sets of primer extension products using said DNA molecule as a template, wherein each of said sets comprises a family of different sized primer extension products terminating at the same base, and further wherein each of said sets comprises the same fluorescent label, wherein the fluorescent labels from at least two of said sets comprise the same donor and acceptor fluorophores in energy transfer relationship separated by different distances and capable of providing distinguishable fluorescence emission patterns, wherein all of the labels of said set are acceptor fluorophores selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes; size separating said primer extension products; detecting said size separated primer extension products; and determining the sequence of said DNA molecule from said detected size separated primer extension products. - View Dependent Claims (2, 3)
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4. A method of sequencing a DNA molecule, said method comprising:
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enzymatically producing a first set of primer extension products terminating in A using a first energy transfer fluorescently labeled oligonucleotide primer comprising a donor fluorophore and an acceptor fluorophore in energy transfer relationship separated by a distance x; enzymatically producing a second set of primer extension products terminating in G using a second energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance y, wherein all of the labels of said set are acceptor flourophores selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluorescein and cyanine dyes; enzymatically producing a third set of primer extension products terminating in T using a third energy transfer fluorescently labeled oligonucleotide primer comprising said donor fluorophore and said acceptor fluorophore in energy transfer relationship separated by a distance z; and enzymatically producing a fourth set of primer extension products terminating in C using a fourth energy transfer fluorescently labeled oligonucleotide primer wherein said donor and acceptor fluorophore are the same; wherein said first through fourth fluorescent labels are capable of providing distinguishable fluorescence emission patterns; combining said four sets of primer extension products; size separating said primer extension products; irradiating said size separated primer extension products whereby each of said side separated product emits a distinguishable fluorescence emission pattern; producing an electropherogram by plotting the intensity of emitted light at two wavelengths as a function of time as each primer extension product passes relative to a detector; and determining the sequence of said DNA molecule from said electropherogram.
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5. A method of determining the presence of at least two different components in a sample, said method comprising:
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labeling a first component of said sample with a first fluorescent label comprising at least one donor and at least one acceptor fluorescer component separated by a distance X; labeling a second component of said sample with a second fluorescent label comprising said at least one donor and said at least one acceptor fluorescer components separated by a distance Y, wherein Y is different from X, wherein all of the labels of said set are acceptor fluorophores selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoroscein and cyanine dyes; irradiating said sample with light at a wavelength absorbed by said acceptor fluorescer component, whereby said first and second labels emit distinguishable fluorescence emission patterns; detecting said distinguishable fluorescence emission patterns; and determining the presence of said two different components from said detection. - View Dependent Claims (6, 7)
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8. A kit for use in DNA sequencing, said kit comprising:
a set of four fluorescently labeled oligonucleotide primers, wherein at least two of said oligonucleotide primers have a common donor and acceptor fluorophore in energy transfer relationship and are separated by different distances, wherein all of the labels of said set are acceptor fluorophores selected from the group consisting of TAM, JOE, FAM, ROX, BODIPY, fluoroscein and cyanine dyes. - View Dependent Claims (9, 10)
Specification