Integrated optic waveguide immunosensor

• US 5,814,565 A
• Filed: 02/23/1995
• Issued: 09/29/1998
• Est. Priority Date: 02/23/1995
• Status: Expired due to Term

• Current Assignee: University of Utah Research Foundation (State of Utah)
• Original Assignee: University of Utah Research Foundation (State of Utah)
• Inventors: Christensen, Douglas A., Wang, Hsu-Kun, Herron, James N., Reichert, W. Monty
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/393,450
• Time in Patent Office: 1,314 Days
• Field of Search: 422/82.05-82.11, 436/525, 385/12
• US Class Current: 422/82.11
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A step-gradient composite waveguide for evanescent sensing in fluorescent binding assays comprises a thick substrate layer having one or more thin film waveguide channels deposited thereon. In one embodiment, the substrate is silicon dioxide and the thin film is silicon oxynitride. Specific binding molecules having the property of binding with specificity to an analyte are immobilized on the surface of the thin film channels. In preferred embodiments, the composite waveguide further includes light input coupling means integrally adapted to the thin film channels. Such light coupling means can be a grating etched into the substrate prior to deposition of the thin film, or a waveguide coupler affixed to the upper surface of the thin film. The waveguide coupler has a thick input waveguide of high refractive index which receives the laser light through one end and propagates it by total internal reflection. The propagated light is then coupled evanescently into the thin film waveguide across a spacer layer of precise thickness and having an index of refraction lower than either the input waveguide or the thin-film waveguide. The composite waveguide can be constructed by plasma vapor deposition of silicon oxynitride onto the silicon dioxide substrate, masking the channel waveguides with a photoresist, and using reactive ion etching to expose the substrate in the unmasked regions.

Claims

1. A kit for performing specific binding assays, which kit includes:

a biosensor comprising;

a step gradient waveguide including a planar substrate formed of a first optical material of refractive index n₁, said planar substrate having a surface disposed adjacent to and in direct contact with a waveguide film formed of a second optical material having a refractive index n₂ which is greater than n₁ ; and

at least one specific binding molecule immobilized to said waveguide film and configured to bind, with specificity, an analyte.

2. The kit of claim 1, wherein said second optical material is selected from the group consisting of:

silicon oxynitride of refractive index n₂, and deposited silicon dioxide; and

said first optical material is selected from the group consisting of;

deposited silicon dioxide, quartz or fused silica, silicon oxynitride of refractive index n₁, and magnesium fluoride.

3. The kit of claim 2, wherein said waveguide film is configured as a plurality of parallel strips of said second optical material spaced from one another along the surface of the planar substrate and constituting a plurality of waveguide channels, and further having a plurality of different species of said specific binding molecules immobilized to said waveguide film, with each of said waveguide channels having a different one of said species immobilized thereto.

4. The kit of claim 2, wherein the surface of the planar substrate further includes a grating formed thereon, said grating being constructed to facilitate coupling of light from an incident excitation beam into said waveguide film.

5. The kit of claim 1, wherein said waveguide film is coated with a coating which provides a level of nonspecific protein binding which is less than about 10% of the amount of specific binding of said analyte, said coating being selected from the group consisting of:

polymethacryloyl polymer, polyethyleneglycol, and avidin; and

wherein said capture molecules are immobilized to said waveguide film by binding to said coating.

6. The kit of claim 1, wherein said step gradient waveguide is manufactured by a process of manufacture including the steps of:

providing a piece of the first optical material having a surface;

vapor depositing the second optical material onto said surface to a depth of between about 0.1 μ

m and 10 mm to produce a waveguide film layer;

coating at least one region of said waveguide film layer with a resist compound which resist compound resists an etchant to produce a coated region and an uncoated region; and

etching said waveguide film layer with an etchant to remove said second optical material from said uncoated region to produce the waveguide film.

7. The kit of claim 6, wherein said second optical material is selected from the group consisting of:

silicon oxynitride of refractive index n₂, and silicon dioxide; and

said first optical material is selected from the group consisting of;

deposited silicon dioxide, quartz or fused silica, silicon oxynitride of refractive index n₁, and magnesium fluoride.

8. The kit of claim 6, in whose manufacture said step of coating at least one region with a resist compound comprises coating a plurality of parallel strips along said surface with said resist compound thereby producing a plurality of waveguide channels, and wherein a plurality of different species of said specific binding molecules are immobilized to said waveguide film, with each of said waveguide channels having a different one of said species immobilized thereto.

9. The kit of claim 8, whose process of manufacture further includes a step of etching a plurality of spaced grooves into said surface of said first optical material prior to said step of depositing said waveguide film layer, wherein said spaced grooves are oriented substantially perpendicular to said plurality of waveguide channels, and wherein spacing of said spaced grooves serves as a grating facilitating efficient coupling of light from an incident excitation beam into said waveguide film.

10. The kit of claim 1, wherein said second optical material is silicon oxynitride having an elemental ratio of approximately Si₂ O₃ N.

11. The kit of claim 1, wherein said step gradient waveguide further includes a waveguide coupler contactingly disposed on said waveguide film, said waveguide coupler comprising:

an input waveguide formed of an optical material having a refractive index n₃, and having a thickness which is between about 0.5 mm and about 5 mm; and

a spacing layer formed of an optical material having a refractive index n₄, wherein n₄ <

n₃ and n₄ <

n₂, and having a thickness selected to optimize the evanescent coupling of light from said input waveguide into said waveguide film.

12. Apparatus for performing evanescent immunofluorescence assays including:

a composite waveguide comprising;

a substrate formed of a first optical material of refractive index n₁ and having two parallel planar surfaces separated by a thickness and a surrounding edge, said thickness being between about 1 μ

m and about 10 mm, and

a waveguide film deposited directly onto at least a portion of one of said two parallel planar surfaces and formed of a second optical material having a refractive index n₂ which is greater than n₁ and a thickness which is between about 0.1 μ

m and about 10 μ

m, said waveguide film having a plurality of specific binding molecules immobilized thereto;

a light source operably disposed to aim a light beam into said composite waveguide for propagation by total internal reflection in said composite waveguide;

light coupling means operably associated with said composite waveguide for facilitating coupling of light beam thereinto;

light collection means positioned for collecting light that impinges on a plane spaced from said two parallel planar surfaces; and

a detector operably disposed with respect to said light collection means to detect the collected light.

13. The apparatus of claim 12, wherein said first and second optical materials are mutually selected from the group of pairwise combinations consisting of:

deposited silicon dioxide/silicon oxynitride;

quartz or fused silica/silicon oxynitride;

silicon oxynitride of refractive index n₁ /silicon oxynitride of refractive index n₂ ; and

magnesium fluoride/deposited silicon dioxide.

14. The apparatus of claim 12, wherein said light coupling means is a waveguide coupler contactingly disposed on said waveguide film, and comprising:

an input waveguide formed of an optical material having a refractive index n₃, and having a thickness which is between about 0.5 mm and about 5 mm; and

a spacing layer formed of an optical material having a refractive index n₄, wherein n₄ <

n₂ and n₄ <

n₃, and having a thickness selected to optimize the evanescent coupling of light from said input waveguide into said waveguide film.

15. The apparatus of claim 13, wherein said waveguide film is constructed to have propagation losses in air of less than about 1 dB/cm.

16. An optical structure for performing specific binding assays with an optical unit comprising a light source positioned to direct light into a waveguide for propagation by total internal reflectance in said waveguide and a detector oriented to detect light from a region proximal to the optical structure, said optical structure comprising:

a substrate comprising a first optical material of refractive index n₁ and having two parallel planar surfaces separated by a thickness and a surrounding edge, the thickness being between about 0.3 μ

m and about 10 mm;

a waveguide film of a second optical material of refractive index n₂, wherein n₂ is greater than n₁, in direct contact with at least a portion of one of said two parallel planar surfaces of said substrate, said waveguide film having a thickness which is between about 0.3 μ

m and about 5 μ

m, and said waveguide film further having a plurality of specific binding molecules immobilized thereto; and

coupling means for coupling light into said waveguide film, said coupling means integrally contacting said waveguide film.

17. The optical structure of claim 16, wherein said second optical material is selected from the group consisting of:

silicon oxynitride of refractive index n₂ and silicon dioxide; and

said first optical material is selected from the group consisting of;

pure silicon dioxide, quartz or fused silica, silicon oxynitride of refractive index n₁, and magnesium fluoride.

18. The optical structure of claim 17, wherein said coupling means is selected from the group consisting of:

an optical grating formed of said first material;

an optical grating formed of said second material; and

a waveguide coupler integrally mounted on said waveguide film, said waveguide coupler comprising

an input waveguide constructed to receive light through an edge and to propagate the received light by total internal reflection, and

a spacing layer interposed between said input waveguide and said waveguide film and having a thickness selected to optimize the evanescent coupling of light from said input waveguide into said waveguide film.

19. The optical structure of claim 18, wherein said first optical material is quartz, fused silica or deposited SiO₂, said coupling means is said waveguide coupler, and wherein said input waveguide is formed of an optical material selected from the group consisting of:

rutile, zirconia, and high-index glass; and

wherein said spacing layer is formed of silicon dioxide and has a thickness of between about 0.1 μ

m and about 5 μ

m.

20. The optical structure of claim 16, wherein said waveguide film is configured as a plurality of strips of said second optical material adhered to one of said two parallel planar surfaces in a spaced parallel array.

21. The optical structure of claim 16, further comprising a plurality of sample wells formed thereon, said sample wells formed by the steps of:

epitaxially depositing an outer layer of refractive index n₁ upon said waveguide film;

applying a resist compound to a plurality of outlines which define said sample wells while leaving areas within and without the outlines uncoated; and

removing said outer layer by etching to expose said waveguide film in said uncoated areas.