Method and an apparatus for determining the content of a constituent of blood of an individual
First Claim
1. A method of determining the content of a constituent of blood of an individual, comprising:
- extracting a whole blood stream of the order of 50-1,000 ml/min from a blood vessel of said individual,directing said whole blood stream through a flow path defining a substantially non-varying flow-through area and comprising a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including opposite first and second optically transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm,propelling said whole blood stream through said flow path by means of a pump causing said whole blood stream to flow through said flow path in a pulsed mode,monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream through said flow-through measuring cuvette,irradiating said first optically transparent surface part of said flow-through measuring cuvette with multi-wavelength near infrared light so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said multi-wavelength near infrared light,detecting the near infrared absorption spectrum represented by near infrared absorption data of said whole blood stream flowing through said flow-through measuring cuvette, andquantifying said content of said constituent by inputting said near infrared absorption data into a mathematical model representing the relation between the near infrared absorption data and the content of said constituent.
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Accused Products
Abstract
A method and an apparatus for determining the content of a constituent of blood of an individual are disclosed. In the method a whole blood stream is extracted from a blood vessel of said individual, the stream being directed through a path defining a substantially non-varying flow-through area wherein is provided a flow-through measuring cuvette including opposite first and second optical transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm. The whole blood stream flowing through the measuring cuvette is irradiated by irradiating the first optical transparent surface part of the measuring cuvette with multi-wavelength near infrared light. The near infrared absorption spectrum is detected and the content of the constituent is quantified on the basis of the detected near infrared absorption data. The method is particularly suited for measuring constituents of whole blood in an extracorporeal loop, for example in hemodialysis.
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Citations
22 Claims
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1. A method of determining the content of a constituent of blood of an individual, comprising:
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extracting a whole blood stream of the order of 50-1,000 ml/min from a blood vessel of said individual, directing said whole blood stream through a flow path defining a substantially non-varying flow-through area and comprising a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including opposite first and second optically transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm, propelling said whole blood stream through said flow path by means of a pump causing said whole blood stream to flow through said flow path in a pulsed mode, monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream through said flow-through measuring cuvette, irradiating said first optically transparent surface part of said flow-through measuring cuvette with multi-wavelength near infrared light so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said multi-wavelength near infrared light, detecting the near infrared absorption spectrum represented by near infrared absorption data of said whole blood stream flowing through said flow-through measuring cuvette, and quantifying said content of said constituent by inputting said near infrared absorption data into a mathematical model representing the relation between the near infrared absorption data and the content of said constituent. - View Dependent Claims (2, 3, 4, 5)
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6. A method of determining the content of a constituent of blood of an individual, comprising:
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extracting a whole blood stream of said individual from a blood vessel of said individual, directing said whole blood stream through a flow path comprising a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including at least one, optically transparent surface part, propelling said whole blood stream through said flow path by means of a pump causing said whole blood stream to flow through said flow path in a pulsed mode, monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream through said flow-through measuring cuvette, irradiating said at least one optically transparent surface part of said flow-through measuring cuvette with electromagnetic radiation of a specific spectral composition, so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said electromagnetic radiation, detecting the electromagnetic radiation absorption spectrum, represented by electromagnetic radiation absorption data, of said whole blood stream flowing through said flow-through measuring cuvette at said periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette, and quantifying said content of said constituent by inputting said electromagnetic radiation absorption data into a mathematical model representing the relation between electromagnetic radiation absorption data and the content of said constituent. - View Dependent Claims (7)
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8. An apparatus for determining the content of a constituent of blood of an individual, comprising:
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means for extracting a whole blood stream from a blood vessel of said individual, a flow path defining a substantially non-varying flow-through area, means for directing said whole blood stream through said flow path, a flow-through measuring cuvette constituting a part of said flow path, said flow-through measuring cuvette including opposite first and second optically transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm, pump means for propelling said whole blood stream through said flow path causing said whole blood stream to flow through said flow path in a pulsed mode, means for monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette, means for generating and irradiating said first optically transparent surface part of said flow-through measuring cuvette with multi-wavelength near infrared light so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said multi-wavelength near infrared light, detector means for detecting the near infrared absorption spectrum of said whole blood stream flowing through said flow-through measuring cuvette represented by near infrared absorption data, and quantifying means for quantifying said content of said constituent by inputting said near infrared absorption data into a mathematical model representing the relation between near infrared absorption data and the content of said constituent. - View Dependent Claims (9, 10, 11, 12)
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13. An apparatus for determining the content of a constituent of blood of an individual, comprising:
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means for extracting a whole blood stream of said individual from a blood vessel of said individual, a flow path, means for directing said whole blood stream through said flow path, a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including at least one optically transparent surface part, means for propelling said whole blood stream through said flow path, causing said flow-path to flow through said flow path in a pulsed mode, monitor means for monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette, means for generating and irradiating said at least one optically transparent surface part of said flow-through measuring cuvette with electromagnetic radiation of a specific spectral composition, so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said electromagnetic radiation of said specific spectral composition, detector means for detecting the electromagnetic radiation absorption spectrum, represented by electromagnetic radiation absorption data, of said whole blood stream flowing through said flow-through measuring cuvette at said periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette, and quantifying means for quantifying said content of said constituent by inputting said electromagnetic radiation absorption data into a mathematical model representing the relation between electromagnetic radiation absorption data and the content of said constituent. - View Dependent Claims (14, 15)
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16. A method of hemodialysis treatment, comprising:
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extracting a whole blood stream of the order of 50-1,000 ml/min from a blood vessel of an individual, directing said whole blood stream through an extracorporeal flow path to a hemodialyser through a flow path comprising a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including opposite first and second optically transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm, propelling said whole blood stream through said flow path by means of a pump causing said whole blood stream to flow through said flow path in a pulsed mode, monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream through said flow-through measuring cuvette, irradiating said first optically transparent surface part of said flow-through measuring cuvette with multi-wavelength near infrared light comprising light of the wavelength range 700-1,800 nm so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said multi-wavelength near infrared light, detecting the near infrared absorption spectrum of said whole blood stream flowing through said flow-through measuring cuvette represented by near infrared absorption data, quantifying the content of urea of said whole blood stream by inputting said near infrared absorption data into a mathematical model representing the relation between near infrared absorption data and the content of urea. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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Specification