Method and an apparatus for determining the content of a constituent of blood of an individual

• US 5,817,007 A
• Filed: 04/26/1996
• Issued: 10/06/1998
• Est. Priority Date: 07/30/1993
• Status: Expired due to Fees

• Current Assignee: Bang & Olufsen Technology A/S, Radiometer Medical ApS (Danaher Corporation)
• Original Assignee: Bang & Olufsen Technology A/S
• Inventors: Fabricius, Paul Erik, Fodgaard, Henrik, Singer, Rolf
• Primary Examiner(s): Bahr, Jennifer
• Assistant Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US08/591,663
• Time in Patent Office: 893 Days
• Field of Search: 128/633, 128/634, 128/664, 356/39-41, 600/310, 600/311, 600/314, 600/315, 600/316, 600/317, 600/322, 600/323, 600/324, 600/325, 600/326, 600/473
• US Class Current: 600/322
• CPC Class Codes: A61B 5/14535 for measuring haematocrit
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: A61B 5/14542 for measuring blood gases A61B5/14551 takes precedence
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/14551 for measuring blood gases
• CPC Class Codes: A61M 1/14 Dialysis systems; Artificial kidneys; Blood oxygenators semi-permeable membranes characterised by the material, manufacturing processes therefor B01D71/00; Reciprocating systems for treatment of body fluids, e.g. single needle systems for hemofiltration or pheresis
• CPC Class Codes: A61M 1/36 Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
• CPC Class Codes: A61M 1/3607 Regulation parameters
• CPC Class Codes: A61M 1/3609 Physical characteristics of the blood, e.g. haematocrit, urea
• CPC Class Codes: A61M 1/367 Circuit parts not covered by the preceding subgroups of group A61M1/3621
• CPC Class Codes: A61M 2205/3306 Optical measuring means
• CPC Class Codes: A61M 2205/3313 used specific wavelengths
• CPC Class Codes: A61M 2205/3317 Electromagnetic, inductive or dielectric measuring means
• CPC Class Codes: A61M 2205/3324 PH measuring means
• CPC Class Codes: A61M 2205/3334 Measuring or controlling the flow rate
• CPC Class Codes: A61M 2205/3375 Acoustical, e.g. ultrasonic, measuring means
• CPC Class Codes: A61M 2205/50 with microprocessors or computers
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 2201/129 : Using chemometrical methods

Abstract

A method and an apparatus for determining the content of a constituent of blood of an individual are disclosed. In the method a whole blood stream is extracted from a blood vessel of said individual, the stream being directed through a path defining a substantially non-varying flow-through area wherein is provided a flow-through measuring cuvette including opposite first and second optical transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm. The whole blood stream flowing through the measuring cuvette is irradiated by irradiating the first optical transparent surface part of the measuring cuvette with multi-wavelength near infrared light. The near infrared absorption spectrum is detected and the content of the constituent is quantified on the basis of the detected near infrared absorption data. The method is particularly suited for measuring constituents of whole blood in an extracorporeal loop, for example in hemodialysis.

Claims

1. A method of determining the content of a constituent of blood of an individual, comprising:

extracting a whole blood stream of the order of 50-1,000 ml/min from a blood vessel of said individual,

directing said whole blood stream through a flow path defining a substantially non-varying flow-through area and comprising a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including opposite first and second optically transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm,

propelling said whole blood stream through said flow path by means of a pump causing said whole blood stream to flow through said flow path in a pulsed mode,

monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream through said flow-through measuring cuvette,

irradiating said first optically transparent surface part of said flow-through measuring cuvette with multi-wavelength near infrared light so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said multi-wavelength near infrared light,

detecting the near infrared absorption spectrum represented by near infrared absorption data of said whole blood stream flowing through said flow-through measuring cuvette, and

quantifying said content of said constituent by inputting said near infrared absorption data into a mathematical model representing the relation between the near infrared absorption data and the content of said constituent.

2. The method according to claim 1, said non-varying flow-through area being of the order of 5-20 mm².

3. The method according to claim 1, said flow-through measuring cuvette comprising a central section including said opposite first and second optically transparent surface parts, tubular inlet and outlet sections and first and second transition sections, said first and second transition sections constituting sections connecting said inlet and outlet sections, respectively, to said central section and presenting gradually changing sectional shapes generating to no substantial extent pressure or velocity gradients to said whole blood stream flowing through said flow-through cuvette so as to eliminate to any substantial extent any blood degradating pressure or velocity impact to said blood flow.

4. The method according to claim 1, said mathematical model being established on the basis of a training set of samples-having relevant known variations in composition and thus producing relevant absorption spectra.

5. The method according to claim 4, said quantifying of said content of said constituent further comprising any of the following mathematical analysis techniques:

multivariate data analysis technique, e.g. PLS analysis technique (Partial Least Square), PCR analysis technique (Principal Components Regression), MLR analysis technique (Multiple Linear Regression) or artificial neural network analysis technique.

6. A method of determining the content of a constituent of blood of an individual, comprising:

extracting a whole blood stream of said individual from a blood vessel of said individual,

directing said whole blood stream through a flow path comprising a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including at least one, optically transparent surface part,

propelling said whole blood stream through said flow path by means of a pump causing said whole blood stream to flow through said flow path in a pulsed mode,

monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream through said flow-through measuring cuvette,

irradiating said at least one optically transparent surface part of said flow-through measuring cuvette with electromagnetic radiation of a specific spectral composition, so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said electromagnetic radiation,

detecting the electromagnetic radiation absorption spectrum, represented by electromagnetic radiation absorption data, of said whole blood stream flowing through said flow-through measuring cuvette at said periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette, and

quantifying said content of said constituent by inputting said electromagnetic radiation absorption data into a mathematical model representing the relation between electromagnetic radiation absorption data and the content of said constituent.

7. The method according to claim 6, said flow-through measuring cuvette comprising opposite first and second optically transparent surface parts and defining a substantially non-varying optical transmission path, and said detection of said electromagnetic radiation absorption spectrum being determined by detecting the transmission of said electromagnetic radiation through said whole blood stream flowing through said flow-through measuring cuvette through said optical transmission path thereof.

8. An apparatus for determining the content of a constituent of blood of an individual, comprising:

means for extracting a whole blood stream from a blood vessel of said individual,

a flow path defining a substantially non-varying flow-through area,

means for directing said whole blood stream through said flow path,

a flow-through measuring cuvette constituting a part of said flow path, said flow-through measuring cuvette including opposite first and second optically transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm,

pump means for propelling said whole blood stream through said flow path causing said whole blood stream to flow through said flow path in a pulsed mode,

means for monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette,

means for generating and irradiating said first optically transparent surface part of said flow-through measuring cuvette with multi-wavelength near infrared light so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said multi-wavelength near infrared light,

detector means for detecting the near infrared absorption spectrum of said whole blood stream flowing through said flow-through measuring cuvette represented by near infrared absorption data, and

quantifying means for quantifying said content of said constituent by inputting said near infrared absorption data into a mathematical model representing the relation between near infrared absorption data and the content of said constituent.

9. The apparatus according to claim 8, said non-varying flow-through area being of the order of 5-20 mm².

10. The apparatus according to claim 8, said flow-through measuring cuvette comprising a central section including said opposite first and second optically transparent surface parts, tubular inlet and outlet sections and first and second transition sections, said first and second transition sections constituting sections connecting said inlet and outlet sections, respectively, to said central section and presenting gradually changing sectional shapes generating to no substantial extent pressure or velocity gradients to said whole blood stream flowing through said flow-through cuvette so as to eliminate to any substantial extent any blood degradating pressure or velocity impact to said blood flow.

11. The apparatus according to claim 8, said mathematical model being established on the basis of a training set of samples having relevant known variations in composition and thus producing relevant absorption spectra.

12. The apparatus according to claim 11, said quantifying of said content of said constituent further comprising any of the following mathematical analysis techniques:

multivariate data analysis technique, e.g. PLS analysis technique (Partial Least Square), PCR analysis technique (Principal Components Regression), MLR analysis technique (Multiple Linear Regression) or artificial neural network analysis technique.

13. An apparatus for determining the content of a constituent of blood of an individual, comprising:

means for extracting a whole blood stream of said individual from a blood vessel of said individual,

a flow path,

means for directing said whole blood stream through said flow path,

a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including at least one optically transparent surface part,

means for propelling said whole blood stream through said flow path, causing said flow-path to flow through said flow path in a pulsed mode,

monitor means for monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette,

means for generating and irradiating said at least one optically transparent surface part of said flow-through measuring cuvette with electromagnetic radiation of a specific spectral composition, so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said electromagnetic radiation of said specific spectral composition,

detector means for detecting the electromagnetic radiation absorption spectrum, represented by electromagnetic radiation absorption data, of said whole blood stream flowing through said flow-through measuring cuvette at said periods of substantially constant flow of said whole blood stream flowing through said flow-through measuring cuvette, and

quantifying means for quantifying said content of said constituent by inputting said electromagnetic radiation absorption data into a mathematical model representing the relation between electromagnetic radiation absorption data and the content of said constituent.

14. The apparatus according to claim 13, said mathematical model being established on the basis of a training set of samples having relevant known variations in composition and thus producing relevant absorption spectra.

15. The apparatus according to claim 14, said quantifying of said content of said constituent further comprising any of the following mathematical analysis techniques:

multivariate data analysis technique, e.g. PLS analysis technique (Partial Least Square), PCR analysis technique (Principal Components Regression), MLR analysis technique (Multiple Linear Regression) or artificial neural network analysis technique.

16. A method of hemodialysis treatment, comprising:

extracting a whole blood stream of the order of 50-1,000 ml/min from a blood vessel of an individual,

directing said whole blood stream through an extracorporeal flow path to a hemodialyser through a flow path comprising a flow-through measuring cuvette constituting part of said flow path, said flow-through measuring cuvette including opposite first and second optically transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm,

propelling said whole blood stream through said flow path by means of a pump causing said whole blood stream to flow through said flow path in a pulsed mode,

monitoring said flow of said whole blood stream through said flow-through measuring cuvette of said flow path, so as to determine periods of substantially constant flow of said whole blood stream through said flow-through measuring cuvette,

irradiating said first optically transparent surface part of said flow-through measuring cuvette with multi-wavelength near infrared light comprising light of the wavelength range 700-1,800 nm so as to expose said whole blood stream flowing through said flow-through measuring cuvette to said multi-wavelength near infrared light,

detecting the near infrared absorption spectrum of said whole blood stream flowing through said flow-through measuring cuvette represented by near infrared absorption data,

quantifying the content of urea of said whole blood stream by inputting said near infrared absorption data into a mathematical model representing the relation between near infrared absorption data and the content of urea.

17. The method according to claim 16, said flow path defining a substantially non-varying flow-through area.

18. The method according to claim 17, said non-varying flow-through area being of the order of 5-20 mm².

19. The method according to claim 16, further comprising continuing said hemodialysis treatment until the content of urea of said whole blood stream has decreased below a specific threshold.

20. The method according to claim 19, said flow-through measuring cuvette comprising a central section including said opposite first and second optically transparent surface parts, tubular inlet and outlet sections and first and second transition sections, said first and second transition sections constituting sections connecting said inlet and outlet sections, respectively, to said central section and presenting gradually changing sectional shapes generating to no substantial extent pressure or velocity gradients to said whole blood stream flowing through said flow-through cuvette so as to eliminate to any substantial extent any blood degradating pressure or velocity impact to said blood flow.

21. The method according to claim 19, said mathematical model being established on the basis of a training set of samples having relevant known variations in composition and thus producing relevant absorption spectra.

22. The method according to claim 21, said quantifying of said content of said constituent further comprising any of the following mathematical analysis techniques:

multivariate data analysis technique, e.g. PLS analysis technique (Partial least Square), PCR analysis technique (Principal Components Regression), MLR analysis technique (Multiple Linear Regression) or artificial neural network analysis technique.