Pullulanase producing microrganisms

• US 5,817,498 A
• Filed: 06/07/1995
• Issued: 10/06/1998
• Est. Priority Date: 12/28/1992
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Amory, Antoine, Deweer, Philippe
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): HOBBS, LISA JOE
• Application Number:
  US08/478,341
• Time in Patent Office: 1,217 Days
• Field of Search: 435/210, 435/252.5
• US Class Current: 435/252.5
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 9/2451 acting on alpha-1,6-glucosidic bonds
• CPC Class Codes: C12N 9/2457 Pullulanase (3.2.1.41)
• CPC Class Codes: C12P 19/16 produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin non-biological hydrolysis of starch C08B30/00
• CPC Class Codes: C12R 2001/07 Bacillus
• CPC Class Codes: C12Y 302/01041 Pullulanase (3.2.1.41)

Abstract

The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the α-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60° C.

The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase.

The invention also relates to the uses thereof and compositions comprising the product.

The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

Claims

1. A biologically pure culture of the species Bacillus deramificans or derivatives or mutants thereof that retain all identifying characteristics of said species, wherein said culture or said derivatives or said mutants produce pullulanase that catalyses hydrolysis of α

-1,6-glucosidic bonds.

2. A biologically pure culture of the strain Bacillus deramificans T 89.117D or derivatives or mutants thereof that retain all identifying characteristics of said strain, wherein said culture or said derivatives or said mutants produce pullulanase that catalyses hydrolysis of α

-1,6-glucosidic bonds.

3. The biologically pure culture of claim 2, wherein said Bacillus deramificans T 89.117D has accession number LMG P-13056.