Genetic immunization
First Claim
1. An pharmaceutical immunizing kit comprising:
- a) a first inoculant comprising;
i) a pharmaceutically acceptable carrier or diluent; and
,ii) a first nucleic acid molecule comprising a nucleotide sequence that encodes at least one HIV protein operatively linked to regulatory sequences;
wherein said nucleotide sequence is capable of being expressed in human cells;
b) a second inoculant comprising;
i) a pharmaceutically acceptable carrier or diluent; and
,ii) a second nucleic acid molecule comprising a nucleotide sequence that encodes at least one HIV protein operatively linked to regulatory sequences;
wherein said nucleotide sequence is capable of being expressed in human cells;
wherein said first nucleic acid molecule is not identical to said second nucleic acid molecule and, taken together, said first nucleic acid molecule and said second nucleic acid molecule encode HIV proteins gag, pol and env; and
c) a third inoculant comprising bupivacaine.
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Accused Products
Abstract
Methods of prophylactic and therapeutic immunization of an individual against pathogen infection, diseases associated with hyperproliferative cells and autoimmune diseases are disclosed. The methods comprise the steps of administering to cells of an individual, a nucleic acid molecule that comprises a nucleotide sequence that encodes a protein which comprises at least one epitope that is identical or substantially similar to an epitope of a pathogen antigen, a hyperproliferative cell associated protein or a protein associated with autoimmune disease respectively. In each case, nucleotide sequence is operably linked to regulatory sequences to enable expression in the cells. The nucleic acid molecule is free of viral particles and capable of being expressed in said cells. The cells may be contacted cells with a cell stimulating agent. Methods of prophylactically and therapeutically immunizing an individual against HIV are disclosed. Pharmaceutical compositions and kits for practicing methods of the present invention are disclosed.
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Citations
34 Claims
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1. An pharmaceutical immunizing kit comprising:
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a) a first inoculant comprising; i) a pharmaceutically acceptable carrier or diluent; and
,ii) a first nucleic acid molecule comprising a nucleotide sequence that encodes at least one HIV protein operatively linked to regulatory sequences;
wherein said nucleotide sequence is capable of being expressed in human cells;b) a second inoculant comprising; i) a pharmaceutically acceptable carrier or diluent; and
,ii) a second nucleic acid molecule comprising a nucleotide sequence that encodes at least one HIV protein operatively linked to regulatory sequences;
wherein said nucleotide sequence is capable of being expressed in human cells;wherein said first nucleic acid molecule is not identical to said second nucleic acid molecule and, taken together, said first nucleic acid molecule and said second nucleic acid molecule encode HIV proteins gag, pol and env; and c) a third inoculant comprising bupivacaine.
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2. A pharmaceutical composition comprising:
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a) a compound selected from the group consisting of;
bupivacaine, mepivacaine, lidocaine, procaine, carbocaine and methyl bupivacaine; andb) a DNA molecule that comprises a DNA sequence that encodes an antigen;
wherein said DNA sequence operatively linked to regulatory sequences which control the expression of said DNA sequence. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. a method of immunizing an individual against an antigen comprising administering to tissue of said individual'"'"'s body,
a) a compound selected from the group consisting of bupivacaine, mepivacaine, lidocaine, procaine, carbocaine and methyl bupivacaine, and b) a DNA molecule that comprises a DNA sequence that encodes said antigen, said DNA sequence operatively linked to regulatory sequences which control the expression of said DNA sequence; wherein said DNA molecule is taken up by cells, said DNA sequence is expressed in said cells and an immune response is generated against said antigen. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
Specification