C-myb ribozymes having 2'-5'-linked adenylate residues

• US 5,817,796 A
• Filed: 05/05/1995
• Issued: 10/06/1998
• Est. Priority Date: 08/26/1992
• Status: Expired due to Fees

• Current Assignee: Dan T. Stinchcomb, James McSwiggen, Kenneth Draper, Thale Jarvis
• Original Assignee: Dan T. Stinchcomb, James McSwiggen, Kenneth Draper, Thale Jarvis
• Inventors: McSwiggen, James, Stinchcomb, Dan T., Jarvis, Thale, Draper, Kenneth
• Primary Examiner(s): LeGuyader, John L.
• Application Number:
  US08/435,628
• Time in Patent Office: 1,250 Days
• Field of Search: 435/91.31, 435/91.1, 435/6, 435/172.1, 435/172.3, 536/23.1, 536/23.2, 536/24.5, 536/25.2, 514/44
• US Class Current: 536/24.5
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 9/08 Vasodilators for multiple indications
• CPC Class Codes: C07K 14/4702 Regulators; Modulating activity
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 15/1135 against oncogenes or tumor suppressor genes
• CPC Class Codes: C12N 15/1136 against growth factors, growth regulators, cytokines, lymphokines or hormones
• CPC Class Codes: C12N 15/1137 against enzymes viral enzymes C12N15/1131; receptors C12N15/1138
• CPC Class Codes: C12N 15/1138 against receptors or cell surface proteins
• CPC Class Codes: C12N 2310/111 spanning the whole gene, or a large part of it
• CPC Class Codes: C12N 2310/121 Hammerhead
• CPC Class Codes: C12N 2310/122 Hairpin
• CPC Class Codes: C12N 2310/123 Hepatitis delta
• CPC Class Codes: C12N 2310/124 based on group I or II introns
• CPC Class Codes: C12N 2310/126 involving RNAse P
• CPC Class Codes: C12N 2310/315 Phosphorothioates
• CPC Class Codes: C12N 2310/317 with an inverted bond, e.g. a cap structure
• CPC Class Codes: C12N 2310/321 2'-O-R Modification
• CPC Class Codes: C12N 2310/322 2'-R Modification
• CPC Class Codes: C12N 2310/332 : Abasic residue
• CPC Class Codes: C12N 2310/335 : Modified T or U
• CPC Class Codes: C12N 2310/3517 : Marker; Tag
• CPC Class Codes: C12N 2310/3521 : Methyl
• CPC Class Codes: C12N 9/6491 : Matrix metalloproteases [MMP's], e.g. interstitial collagenase (3.4.24.7); Stromelysins (3.4.24.17; 3.2.1.22); Matrilysin (3.4.24.23)

Abstract

Enzymatic nucleic acid molecules which cleave c-myb RNA or other RNAs associated with restenosis or cancer.

Claims

1. An enzymatic RNA molecule for the specific cleavage of c-myb RNA, wherein said enzymatic RNA molecule comprises an oligonucleotide having a 2'"'"'-5'"'"'-linked adenylate residue having a 5'"'"'-phosphate.

2. The enzymatic RNA molecule of claim 1, wherein said enzymatic RNA molecule is complementarity to c-myb RNA at a region selected from the group consisting of:

SEQ ID Nos. 1-100, 130-148, and even numbered SEQ ID Nos. from SEQ ID No. 170 to 1224.

3. The enzymatic RNA molecule of claim 1, wherein said enzymatic RNA molecule is complementarity to c-myb RNA at a region other than the region consisting of nucleotides 111-140.