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Alkaline phosphatase

  • US 5,821,095 A
  • Filed: 01/12/1996
  • Issued: 10/13/1998
  • Est. Priority Date: 01/12/1995
  • Status: Expired due to Fees
First Claim
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1. An alkaline phosphatase having the following physical and chemical properties:

  • (1) the alkaline phosphatase catalyzes the following reaction;

    orthophosphoric acid monoester+H2 O→

    alcohol+orthophosphoric acid;

    (2) activators selected from the group consisting of Mg++ and Co++ ;

    (3) stabilizers selected from the group consisting of Mg++ and Co++ ;

    (4) a thermal stability wherein the alkaline phosphatase is stable at least for 30 minutes when treated at pH 7.5 and 60°

    C.;

    (5) a specific activity of at least 2,300 U/mg;

    (6) the alkaline phosphatase has no sugar chain; and

    (7) a gel filtration molecular weight from about 140,000 to about 150,000 and a SDS-PAGE molecular weight from about 65,000 to about 67,000.

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