Propargylethoxyamino nucleotides
First Claim
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1. A nucleoside compound having the structure:
- ##STR12## wherein;
R1 and R2 taken separately are selected from the group consisting of --H, lower alkyl, protecting group, and label;
B is a 7-deazapurine, purine, or pyrimidine nucleoside base;
wherein when B is purine or 7-deazapurine, the sugar moiety is attached at the N9 -position of the purine or deazapurine, and when B is pyrimidine, the sugar moiety is attached at the N1 -position of the pyrimidine; and
wherein when B is a purine, the adjacent triple-bonded carbon is attached to the 8-position of the purine, when B is 7-deazapurine, the adjacent triple-bonded carbon is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, the adjacent triple-bonded carbon is attached to the 5-position of the pyrimidine;
W1 is selected from the group consisting of --H and --OH;
W2 is --OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'"'"'-position; and
W3 is selected from the group consisting of --PO4, --P2 O7, --P3 O10, phosphate analog, and --OH.
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Abstract
Propargylethoxyamino nucleosides are disclosed having the structure ##STR1## wherein R1 and R2 are --H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is --H or --OH; W2 is --OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'"'"'-position; and W3 is --PO4, --P2 O7, --P3 O10, phosphate analog, or --OH. Additionaly, a primer extension method is provided employing the above propargylethoxyamino nucleosides.
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Citations
8 Claims
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1. A nucleoside compound having the structure:
- ##STR12## wherein;
R1 and R2 taken separately are selected from the group consisting of --H, lower alkyl, protecting group, and label;B is a 7-deazapurine, purine, or pyrimidine nucleoside base; wherein when B is purine or 7-deazapurine, the sugar moiety is attached at the N9 -position of the purine or deazapurine, and when B is pyrimidine, the sugar moiety is attached at the N1 -position of the pyrimidine; and wherein when B is a purine, the adjacent triple-bonded carbon is attached to the 8-position of the purine, when B is 7-deazapurine, the adjacent triple-bonded carbon is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, the adjacent triple-bonded carbon is attached to the 5-position of the pyrimidine; W1 is selected from the group consisting of --H and --OH; W2 is --OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'"'"'-position; and W3 is selected from the group consisting of --PO4, --P2 O7, --P3 O10, phosphate analog, and --OH. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- ##STR12## wherein;
Specification