Flow cytometer

• US 5,824,269 A
• Filed: 12/22/1995
• Issued: 10/20/1998
• Est. Priority Date: 12/26/1994
• Status: Expired due to Term

• Current Assignee: Toa Medical Electronics Co., Ltd.
• Original Assignee: Toa Medical Electronics Co., Ltd.
• Inventors: Kubota, Fumio, Nakamoto, Hiroyuki, Kosaka, Tokihiro
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US08/577,241
• Time in Patent Office: 1,033 Days
• Field of Search: 422/73, 422/81, 422/82.05, 436/52, 436/63, 250/461.2, 382/133, 382/134, 356/39, 356/73, 356/317, 356/318, 356/337, 356/338, 364/555
• US Class Current: 422/73
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream

Abstract

A flowcytometer includes a sheath flow cell for forming a sample stream containing particles; a detector for detecting the particle at a first area to generate a signal representative of the particle; an imaging device for capturing an image of the particle at a second area of the sample stream; a display; a calculator for calculating a plurality of characteristic parameters of each particle; a distribution preparation device for preparing a distribution of the characteristic parameters to display the distribution on the display; a designation device for previously designating a region in the distribution; a region memory for storing the designated region; a decision device for deciding whether the characteristic parameters of the particle detected at the first area by the detection means are located in the designated region stored in the region memory; an image controller for allowing the imaging device to imaging the particle when the characteristic parameters are located in the designated region; an image memory for storing the image of the particle; and a controller for selectively reading the image of the particle to allow the display device to display the read image.

Claims

1. The flow cytometer in combination comprising:

a sheath flow cell for forming a sample stream containing particles;

detection means for detecting a particle at a first area of the sample stream to generate a signal representative of the particle;

imaging means operatively connected to the detection means for imaging the particle at a second area of the sample stream, the second area being a different area than the first area;

display means for displaying a read image of the particle;

calculation means for calculating a plurality of characteristic parameters indicating characteristics of the particle based on the generated signal;

distribution preparation means for preparing a distribution of the characteristic parameters and displaying the distribution on the display means;

region designation means for designating at least one predetermined region in the distribution;

region storage means for storing the at least one designated predetermined region;

decision means for deciding whether the characteristic parameters of the particle detected at the first area by the detection means are located in the at least one designated predetermined region stored in the region storage means;

imaging control means for allowing the imaging means to image the particle when the characteristic parameters are located in the at least one designated predetermined region;

image storage means for storing the image of the particle obtained by the imaging means; and

displaying control means for selectively reading the image of the particle to form a read image and for allowing the display means to display the read image.

2. The flow cytometer of claim 1,

wherein the distribution preparation means prepares a plurality of distributions based on the characteristic parameters of the particle designated by parameter designation means and displays the plurality of distributions on the display means,

the region designation means designates a predetermined region in each distribution, and

the imaging means images the particle when the plurality of characteristic parameters for the particle detected by the detection means are located in the designated regions of the plurality of distributions.

3. The flow cytometer of claim 1,

wherein the region designation means designates a plurality of regions in a distribution, and

the imaging means images the particle when the plurality of characteristic parameters for the particle detected by the detection means are located in any one of the designated regions of the distribution.

4. The flow cytometer of claim 1,

wherein the region storage means stores the at least one designated predetermined region by allowing memory addresses thereof to correspond to the characteristic parameters of the particle so that data bits inside the at least one designated predetermined region and data bits outside the at least one designated predetermined region are represented by different binary data, and

the decision means decides whether the characteristic parameters of the particle are located in the at least one designated predetermined region based on the binary data.

5. The flow cytometer of claim 1 further comprising means for presetting the number of imaging times for the imaging means with respect to each predetermined region designated by the region designation means.

6. The flow cytometer of claim 1, wherein the imaging control means comprises triggering signal generating means for generating a signal every time the particle reaches the second area and based on the generated signal, serves to trigger the imaging means.

7. The flow cytometer of claim 1, further comprising coordinate designation means for designating a coordinate point in the distribution,

wherein the displaying control means, when the coordinate designation means designates a coordinate point corresponding to the imaged particle, reads the image of the particle corresponding to the designated coordinate point to allow the display means to display the read image of the particle.

8. The flow cytometer of claim 7,

wherein the coordinate point corresponding to the imaged particle is represented with a first identifier in the distribution.

9. The flow cytometer of claim 7,

wherein the coordinate point corresponding to the the read image of the particle is represented with a second identifier in the distribution.

10. The flow cytometer of claim 1, wherein the detection means includes means for optically detecting the particle.

11. The flow cytometer of claim 10, wherein the imaging means includes a light source.