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Detection of mismatches by cleavage of nucleic acid heteroduplexes

  • US 5,824,471 A
  • Filed: 06/05/1995
  • Issued: 10/20/1998
  • Est. Priority Date: 06/05/1995
  • Status: Expired due to Fees
First Claim
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1. A method of detecting a mismatch between a first nucleic acid strand and a second nucleic acid strand comprising:

  • forming a plurality of duplexes, each duplex including a molecule of said first nucleic acid strand, or a portion thereof, and a molecule of said second nucleic acid strand, or a portion thereof;

    contacting a first bacteriophage resolvase and a second bacteriophage resolvase with said plurality of duplexes, wherein said first and said second resolvases are different;

    determining if said plurality of duplexes is cleaved by either or both of said first and said second resolvases, wherein cleavage indicates the presence of a mismatch.

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