Nucleic acid mediated electron transfer
First Claim
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1. A method of detecting a target sequence in a nucleic acid sample comprising:
- a) initiating electron transfer by applying an initiation signal to a hybridization complex comprising said target sequence, if present, hybridized to a single-stranded probe nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, wherein said electron donor moiety and said electron acceptor moiety are covalently attached to said probe nucleic acid, and wherein said electron donor moiety and said electron acceptor moiety are not redox proteins; and
b) detecting electron transfer between said electron donor and said electron acceptor moieties as an indication of the presence or absence of said target sequence.
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Abstract
The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
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Citations
22 Claims
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1. A method of detecting a target sequence in a nucleic acid sample comprising:
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a) initiating electron transfer by applying an initiation signal to a hybridization complex comprising said target sequence, if present, hybridized to a single-stranded probe nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, wherein said electron donor moiety and said electron acceptor moiety are covalently attached to said probe nucleic acid, and wherein said electron donor moiety and said electron acceptor moiety are not redox proteins; and b) detecting electron transfer between said electron donor and said electron acceptor moieties as an indication of the presence or absence of said target sequence. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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2. A method of detecting a target sequence in a nucleic acid sample comprising:
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a) initiating electron transfer by applying an initiation signal to a hybridization complex comprising said target sequence, if present, hybridized to a single-stranded probe nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, wherein at least one of said electron donor moiety and said electron acceptor moiety is an electrode and the other is an electron transfer moiety which is not a redox protein and which is covalently attached to said nucleic acid; and b) detecting electron transfer between said electron donor and said electron acceptor moieties as an indication of the presence or absence of said target sequence.
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3. A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, wherein said method comprises:
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a) initiating electron transfer by applying an initiation signal to a hybridization complex comprising a first probe nucleic acid containing at least one covalently attached electron donor moiety hybridized to said first target domain and a second probe nucleic acid containing at least one covalently attached electron acceptor moiety hybridized to said second target domain, wherein said electron donor and acceptor moieties are not redox proteins; and b) detecting electron transfer between said electron donor and said electron acceptor moieties. - View Dependent Claims (22)
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4. A method of detecting a target sequence in a nucleic acid wherein said target sequence comprises a first target domain and a second target domain adjacent to said first target domain, wherein said method comprises:
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a) initiating electron transfer by applying an initiation signal to a hybridization complex comprising a first probe nucleic acid containing at least one electron donor moiety hybridized to said first target domain and a second probe nucleic acid containing at least one electron acceptor moiety hybridized to said second target domain, wherein at least one of said electron donor moiety and said electron acceptor moiety is an electrode, and the other is an electron transfer moiety which is not a redox protein and which is covalently attached to said nucleic acid; and b) detecting electron transfer between said electron donor and said electron acceptor moieties.
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Specification