Dry reagent test strip comprising benzidine dye precursor and antipyrine compound

• US 5,824,491 A
• Filed: 05/17/1996
• Issued: 10/20/1998
• Est. Priority Date: 05/17/1996
• Status: Expired due to Fees

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Mercury Diagnostics, Inc.
• Inventors: Nelson, Eric Michael, Priest, John Hedger
• Primary Examiner(s): Leary, Louise N.
• Application Number:
  US08/649,777
• Time in Patent Office: 886 Days
• Field of Search: 435/14, 435/25, 435/28, 435/975, 435/970, 422/56, 422/57, 548/360.7, 534/822
• US Class Current: 435/28
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: C12Q 2326/12 3,3',5,5'-Tetramethylbenzidine, i.e. TMB
• CPC Class Codes: C12Q 2326/14 Ortho-Tolidine, i.e. 3,3'-dimethyl-(1,1'-biphenyl-4,4'-diamine)
• CPC Class Codes: C12Q 2326/96 4-Amino-antipyrine
• CPC Class Codes: Y10S 435/97 Test strip or test slide

Abstract

A dry reagent test strip for determining the concentration of an analyte in a liquid sample is described. The test strip has a matrix containing reagent detection chemistry, which includes an oxidase enzyme that can utilize the analyte as a substrate forming hydrogen peroxide, a benzidine dye precursor, a peroxidase enzyme, and an antipyrine compound. The addition of an antipyrine compound to the reagent detection chemistry provides a standard concentration graph which is substantially linear in a desired range of analyte concentration. The precision and accuracy of reading the test strip are enhanced.

Claims

1. A dry reagent test strip for determining the concentration of an analyte in a liquid sample, the test strip comprising a matrix containing reagent detection chemistry, the reagent detecting chemistry comprising:

an oxidase enzyme that utilizes the analyte as a substrate forming hydrogen peroxide;

a benzidine dye precursor;

a peroxidase enzyme; and

an antipyrine compound, wherein the antipyrine compound produces a standard curve of analyte concentration versus reflectance which is substantially linear in the desired range when the analyte contacts the reagent detecting chemistry.

2. The strip of claim 1, wherein the benzidine dye precursor is o-tolidine.

3. The strip of claim 1, wherein the benzidine dye precursor is o-tolidine HCl.

4. The strip of claim 1, wherein the benzidine dye precursor is a tetralkylbenzidine.

5. The strip of claim 4, wherein the benzidine dye precursor is a tetramethylbenzidine.

6. The strip of claim 1, wherein the antipyrine compound is an aminoantipyrine.

7. The strip of claim 6, wherein the aminoantipyrine is 4-aminoantipyrine.

8. A dry reagent test strip for determining the concentration of glucose in a sample of whole blood, the test strip comprising a matrix containing reagent detection chemistry, the reagent detecting chemistry comprising:

glucose oxidase, a benzidine dye precursor, peroxidase and an antipyrine compound , wherein the antipyrine compound produces a standard curve of glucose concentration versus reflectance which is substantially linear in the desired range when the glucose contacts the reagent detecting chemistry.

9. The strip of claim 8, wherein the antipyrine compound is an aminoantipyrine.

10. The strip of claim 9, wherein the aminoantipyrine is 4-aminoantipyrine.

11. The strip of claim 8, wherein the benzidine dye precursor is o-tolidine.

12. The strip of claim 8, wherein the benzidine dye precursor is o-tolidine HCl.

13. The strip of claim 8, wherein the benzidine dye precursor is tetraalkylbenzidine.

14. The strip of claim 13, wherein the benzidine dye precursor is tetramethylbenzidine.