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Process for the production of expression vectors comprising at least one stochastic sequence of polynucleotides

DC
  • US 5,824,514 A
  • Filed: 06/05/1995
  • Issued: 10/20/1998
  • Est. Priority Date: 03/30/1985
  • Status: Expired due to Term
First Claim
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1. A process for the production of an expression vector which comprises at least one stochastic sequence of polynucleotides, comprising the steps of:

  • providing in an appropriate buffer at least three different sequences of oligonucleotides, said oligonucleotides each comprising at least 7 nucleotide residues;

    polymerizing said oligonucleotides to form a stochastic sequence of polynucleotides; and

    ligating said stochastic sequence of polynucleotides into a linearized expression vector.

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