Method for detecting and quantitating nucleic acid impurities in biochemical preparations

• US 5,824,557 A
• Filed: 04/02/1996
• Issued: 10/20/1998
• Est. Priority Date: 04/02/1996
• Status: Expired due to Term

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: PanVera Corp. (Vertex Pharmaceuticals Incorporated)
• Inventors: Burke, Thomas J., Bolger, Randall E., Lenoch, Francis J.
• Primary Examiner(s): Horlick, Kenneth R.
• Application Number:
  US08/626,520
• Time in Patent Office: 931 Days
• Field of Search: 436/94, 435/6, 435/810
• US Class Current: 436/94
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/173 staining/intercalating agent, e.g. ethidium bromide
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A homogeneous fluorescence-based nucleic acid detection and quantitation system is provided to measure nucleic acid in protein solutions. The process relies on the intercalation of a non-fluorescent dye into a double-stranded nucleic acid helix or single-stranded nucleic acid. The dye fluoresces after intercalation and the intensity is a direct measurement of the amount of nucleic acid present in the sample.

Claims

1. A process for detecting and quantitating an amount of a nucleic acid in a protein solution, comprising:

a) measuring at least one light-emitting characteristic of a plurality of test solutions, each having a different known amount of nucleic acid and each having a nucleic acid detecting label;

b) forming a curve based on the light-emitting characteristic measurements of step a);

c) adding the label to a sample of the protein solution having a detergent and measuring at least one light-emitting characteristic from the sample; and

,

d) comparing the light-emitting characteristic measurements of step c) with the curve and determining the amount of nucleic acid in the sample.

2. The process of claim 1 wherein the label is a light-emitting compound.

3. The process of claim 2 wherein the light-emitting compound intercalates with nucleic acid and emits light more strongly when intercalating with double-stranded nucleic acid and less strongly when intercalating with single-stranded nucleic acid.

4. The process of claim 3 wherein the light-emitting compound is a fluorescence-emitting dye which is selected from the group consisting of PicoGreen™

, acridine orange, YOYO I, PanVera Dye and ethidium bromide.

5. The process of claim 1 wherein the light-emitting characteristic is selected from the group consisting of intensity and polarization.

6. The process of claim 1 wherein the detergent is selected from the group consisting of SDS, alky sulfates, bile acids and quaternary ammonium detergents.

7. The process of claim 1 wherein the nucleic acid is selected from the group consisting of DNA and RNA.

8. The process of claim 1 further comprising detecting and quantitating an amount of a nucleic acid in a protein solution for monitoring nucleic acid removal of a recombinant protein manufacture.

11. The process of claim 1 further comprising the assay buffer having an amount of inherent fluorescence and DNA material that is negligible when obtaining the quantity of DNA present in the sample.

9. A process for monitoring nucleic acid removal during recombinant protein manufacture, comprising:

a) adding a detergent to a sample of protein solution in the presence of a purified assay buffer;

b) adding a dye that strongly emits fluorescence when intercalating with double-stranded DNA;

c) measuring an intensity from the dye;

d) comparing the intensity from step c) with a curve for obtaining a quantity of DNA present in the sample.

10. The process of claim 9 wherein the detergent is selected from the group consisting of SDS, alky sulfates, bile acids and quaternary ammonium detergents.

12. A kit for detecting and quantitating an amount of nucleic acid present in a protein sample, comprising:

a) a receptacle containing a chemical label for detecting and quantitating an amount of nucleic acid present in a sample;

b) a receptacle containing a purified assay buffer;

c) a receptacle containing a nucleic acid standard for forming a curve; and

,

d) a receptacle containing a detergent for preventing nucleic acid binding to protein.

13. The kit of claim 12 further comprising a receptacle containing a known amount of double-stranded nucleic acid covalently bound to a polarizationly readable label.