Blue light photoreceptors and methods of using the same

• US 5,824,859 A
• Filed: 07/08/1994
• Issued: 10/20/1998
• Est. Priority Date: 07/08/1994
• Status: Expired due to Fees

• Current Assignee: Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Original Assignee: Trustees Of The University Of Pennsylvania (University of Pennsylvania)
• Inventors: Ahmad, Margaret, Cashmore, Anthony Robert, Lin, Chentao
• Primary Examiner(s): Benzion, Gary
• Application Number:
  US08/272,255
• Time in Patent Office: 1,565 Days
• Field of Search: 435/172.3, 435/172.1, 435/320.1, 800/205, 536/23.2, 536/23.6, 935/64
• US Class Current: 800/290
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8241 Phenotypically and genetically modified plants via recombinant DNA technology

Abstract

The invention features a substantially pure preparation of a nucleic acid encoding a HY4 or a HY4-related gene. The invention further features transgenic plants encoding a HY4 gene having a shorter stem than substantially homozygous wild type nontransgenic plants; and, transgenic plants comprising complementary HY4 sequences having a longer stem than substantially homozygous wild type nontransgenic plants.

Claims

1. A substantially pure preparation of a nucleic acid specifying the sequence of a plant HY4 gene, said gene comprising all mutants, derivatives or homologs of SEQ ID #5 which retain the activity of modifying stem length in a plant.

3. The nucleic acid of claim 1 comprising the DNA sequence of Arabidopsis HY4.

4. The nucleic acid of claim 1 comprising the DNA sequence of Oryza sativa HY4.

5. The nucleic acid of claim 1 comprising the DNA sequence of Pisum sativum HY4.

6. The nucleic acid of claim 3 which encodes the amino acid sequence of Arabidopsis HY4.

7. The nucleic acid of claim 4 which encodes the amino acid sequence of Oryza sativa HY4.

8. The nucleic acid of claim 5 which encodes the amino acid sequence of Pisum sativum HY4.

9. A recombinant vector comprising the nucleic acid of claim 1.

10. A recombinant cell comprising the nucleic acid of claim 1.

2. A substantially pure preparation of a nucleic acid encoding a plant HY4 polypeptide, said polypeptide comprising all mutants, derivatives or homologs of SEQ ID #6 which retain the activity of modifying stem length in a plant.

11. A transgenic plant, the cells of said plant comprising a substantially pure preparation of the nucleic acid of SEQ ID NO:

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

12. The transgenic plant of claim 11, the seeds of said plant comprising a substantially pure preparation of the nucleic acid of SEQ ID NO:

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

13. The transgenic plant of claim 11, the progeny of said plant comprising a substantially pure preparation of the nucleic acid of SEQ ID NO:

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

14. The transgenic plant of claim 11, wherein said nucleic acid is overexpressed in cells of said plant when compared with expression of said nucleic acid in a nontransgenic but otherwise substantially homozygous plant.

15. The transgenic plant of claim 14, the stem of said plant being shorter than the stem of a nontransgenic but otherwise substantially homozygous wild type plant.

16. The transgenic plant of claim 15, wherein said plant is selected from the group consisting of Oryza sativa, Avena spp, Triticum spp, Hordeum vulgare, Saccharum spp, Zea mays, Secale cereale, Glycine max, Lycopersicon esculentum, Zea mays and Sorghum bicolor.

17. A method of generating a transgenic plant having a substantially pure preparation of the nucleic acid of SEQ ID NO:

5, said plant comprising a shorter stem than an otherwise substantially homozygous wild type plant, comprising introducing into cells of said transgenic plant the nucleic acid of SEQ ID NO;

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

18. A substantially pure preparation of a nucleic acid complementary to a portion of the plant HY4 gene of SEQ ID #5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in a plant, wherein said nucleic acid is capable of inhibiting expression of said HY4 gene when introduced into cells comprising said HY4 gene.

20. The nucleic acid of claim 18 or 19, comprising a sequence complementary to Arabidopsis HY4 nucleic acid.

21. The nucleic acid of claim 18 or 19, comprising a sequence complementary to Oryza sativa nucleic acid.

22. The nucleic acid of claim 18 or 19, comprising a sequence complementary to Pisum sativum HY4 nucleic acid.

23. A recombinant vector comprising the nucleic acid of claim 18 or 19.

24. A recombinant cell comprising the nucleic acid of claim 18 or 19.

19. A substantially pure preparation of a nucleic acid complementary to the plant HY4 gene of SEQ ID #5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in a plant, wherein said nucleic acid is capable of inhibiting expression of said HY4 gene when introduced into cells comprising said HY4 gene.

25. A transgenic plant, the cells of said plant comprising a recombinant transgene comprising a nucleic acid complementary to a plant HY4 gene, said HY4 gene being SEQ ID NO:

5, or any portion, mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

27. The transgenic plant of claim 25, the seeds of said plant comprising a recombinant transgene comprising a nucleic acid complementary to a plant HY4 gene, said HY4 gene being SEQ ID NO:

5, or any portion, mutants, derivatives or variants thereof which retain activity of modifying stem length in said plant.

29. The transgenic plant of claim 25, the progeny of said plant comprising a recombinant transgene comprising a nucleic acid complementary to a plant HY4 gene, said HY4 gene being SEQ ID NO:

5, or any portion, mutants, derivatives or variants thereof which retain the activity of modifying stem length in said plant.

31. The transgenic plant of claim 25 or 26, the stem of said plant being longer than the stem of a nontransgenic but otherwise substantially homozygous wild type plant.

34. The transgenic plant of claim 25 or 26, wherein said plant is selected from the group consisting of Camellia sinensis, Vitis spp, Gossypium spp, Pinus radiata and Populus trichocarpa.

26. A transgenic plant, the cells of said plant comprising a recombinant transgene comprising a nucleic acid complementary to a HY4 gene, said HY4 gene being SEQ ID NO:

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

28. The transgenic plant of claim 26, the seeds of said plant comprising a recombinant transgene comprising a nucleic acid complementary to a plant HY4 gene, said HY4 gene being SEQ ID NO:

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

30. The transgenic plant of claim 26, the progeny of said plant comprising a recombinant transgene comprising a nucleic acid complementary to a plant HY4 gene, said HY4 gene being SEQ ID NO:

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said plant.

32. A method of inhibiting expression of HY4 in a cell comprising introducing into said cell a recombinant transgene comprising a nucleic acid complementary to a plant HY4 gene, said HY4 gene being SEQ ID NO:

5, or any portion, mutants, derivatives or variants thereof which retain the activity modifying stem length in a plant.

33. A method of generating a transgenic plant comprising a longer stem than an otherwise substantially homozygous wild type plant, comprising introducing into cells of said transgenic plant a recombinant transgene comprising a nucleic acid complementary to a plant HY4 gene, said HY4 gene being SEQ ID NO:

5, or any mutants, derivatives or homologs thereof which retain the activity of modifying stem length in said transgenic plant.