Method and apparatus for determining analytical data concerning the inside of a scattering matrix
First Claim
1. A method for determining analytical data regarding a light scattering matrix, said method comprising the steps of:
- detecting secondary light by irradiating light into the scattering matrix as primary light at an irradiation site through an interface bounding the scattering matrix, and detecting light emerging out of the scattering matrix through the interface, said emerging light being detected as secondary light at a detection site which is located at a predetermined measuring distance from the irradiation site;
determining a measurement value of a measurable physical property of the secondary light, said measurable physical property varying due to an interaction of the primary light with the scattering matrix;
evaluating the measurement value as a measure of the analytical data to be determined, the analytical data being determined based upon a measurement value of the measurement variable,wherein at least two of said detecting steps are carried out under different reflection conditions at the interface between the irradiation site and the detection site, and measurement values determined in said at least two detection steps are evaluated in said evaluation step to determine said analytical data.
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Abstract
A method and an apparatus for determining analytical data concerning the inside of a scattering matrix, in particular of a biological sample. In a detection step detection measurements are carried out in which light is irradiated into the matrix as primary light at an irradiation site (12) through an interface bounding the scattering matrix (6) and light emerging out of the scattering matrix through the interface is detected as secondary light at a detection site (13) at a predetermined measuring distance from the irradiation site, in order to determine as a measurement variable a measurable physical property of the light which varies due to the interaction of the light with the scattering matrix, which measurement variable is a measure of the analytical data to be determined. In an evaluation step the analytical data are determined on the basis of the measurement variable measured in the detection step. In order that such a matrix analysis may be carried out with relatively simple measuring means, at least two detection measurements are carried out in the detection step with different reflection conditions at the interface (5) between the irradiation site (12) and the detection site (13), in each of which the measurement value of the measurement variable is determined.
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Citations
21 Claims
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1. A method for determining analytical data regarding a light scattering matrix, said method comprising the steps of:
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detecting secondary light by irradiating light into the scattering matrix as primary light at an irradiation site through an interface bounding the scattering matrix, and detecting light emerging out of the scattering matrix through the interface, said emerging light being detected as secondary light at a detection site which is located at a predetermined measuring distance from the irradiation site; determining a measurement value of a measurable physical property of the secondary light, said measurable physical property varying due to an interaction of the primary light with the scattering matrix; evaluating the measurement value as a measure of the analytical data to be determined, the analytical data being determined based upon a measurement value of the measurement variable, wherein at least two of said detecting steps are carried out under different reflection conditions at the interface between the irradiation site and the detection site, and measurement values determined in said at least two detection steps are evaluated in said evaluation step to determine said analytical data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An apparatus for determining analytical data of a light scattering matrix, said apparatus comprising:
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a measuring head, said measuring head including a sample contact surface for contacting an interface of the biological sample; light irradiation means disposed in said measuring head for irradiating light into the biological sample at an irradiation site through an irradiation opening in the sample contact surface, detection means connected to said measuring head for detecting secondary light emerging from the scattering matrix at a detection site through a detection opening in the sample contact surface; evaluation means connected to said light irradiation means and said detection means for determining analytical data based upon the primary light and the secondary light, wherein said sample contact surface includes reflection adjustment means wherein different reflection conditions can be provided in at least two detection measurements. - View Dependent Claims (19, 20, 21)
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Specification