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Methods and composition for in vivo gene therapy

  • US 5,827,703 A
  • Filed: 05/19/1994
  • Issued: 10/27/1998
  • Est. Priority Date: 06/04/1992
  • Status: Expired due to Fees
First Claim
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1. A method of introducing a DNA expression cassette into cells of a mammal, said method comprising introducing the expression cassette into the manual systemically, wherein:

  • the DNA expression cassette comprises a promoter, and a DNA sequence encoding a gene product;

    the DNA expression cassette is complexed to a lipid carrier comprising cationic lipids and cholesterol having a mean diameter of less than about 10 microns, resulting in a DNA expression cassette-lipid carrier complex;

    the DNA expression cassette and lipid carrier does not aggregate in vitro; and

    the DNA expression cassette to lipid carrier ratio is less than 6;

    1 micrograms DNA to nanomoles cationic lipid.

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