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Process for efficiently producing transglutaminase through DNA recombination

  • US 5,827,712 A
  • Filed: 05/17/1996
  • Issued: 10/27/1998
  • Est. Priority Date: 05/17/1995
  • Status: Expired due to Term
First Claim
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1. A process for producing a transglutaminase, which comprises incubating E. coli comprising a gene encoding a heat shock protein DnaJ and a gene encoding a transglutaminase, wherein each of said genes is operably linked in proper reading frame to sequences which direct the expression of each gene such that expression of the DnaJ gene is enhanced and the transglutaminase produced is biologically active and is present in soluble form at a level higher than that of transglutaminase expressed in E. coli in which expression of the DnaJ gene is not enhanced, and collecting the transglutaminase from said E. coli.

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