Chemical sensor using two-dimensional lens array

• US 5,827,748 A
• Filed: 01/24/1997
• Issued: 10/27/1998
• Est. Priority Date: 01/24/1997
• Status: Expired due to Fees

• Current Assignee: Navy The United Stated of America As Represented By The Secretary of The
• Original Assignee: the united states of america as represented by the secretary of the navy
• Inventors: Golden, Joel P.
• Primary Examiner(s): Spiegel, Carol A.
• Application Number:
  US08/787,720
• Time in Patent Office: 641 Days
• Field of Search: 422/82.05-82.11, 435/7.1, 435/7.9, 435/968, 435/970, 435/973, 436/518, 436/172, 436/807, 436/809, 436/527, 436/806
• US Class Current: 436/527
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10S 435/968 High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

A chemical sensor includes a patterned layer having discrete sections, a -dimensional detector array, and a two-dimensional lens array that focuses an optical signal from the patterned layer onto the two dimensional detector array. Typically, the two-dimensional detector array is a charge-coupled device array and the lens array is a graded index of refraction lens array. The chemical sensor maintains good resolution throughout its field of view.

Claims

1. A chemical sensor for detecting a plurality of analytes in a sample, said sensor comprising:

a patterned signal layer comprising a two-dimensional array of discrete, optically transparent test sections at known locations thereon, each of said test sections comprising an immobilized specific binding partner for one of said plurality of analytes, such that specific binding of said analyte to said immobilized specific binding partner generates an optical signal in said test section indicative of the presence of said analyte;

a two-dimensional light sensor array which detects said optical signal in a corresponding discrete detection section; and

a two-dimensional array of graded index of refraction lenses which focuses each said optical signal from each said test section into each said corresponding detection section.

2. The chemical sensor of claim 1 wherein said patterned signal layer is a waveguide comprising said discrete test sections of said immobilized specific binding partners.

3. The chemical sensor of claim 2 wherein said waveguide is a glass coverslip or a microscope slide.

4. The chemical sensor of claim 1 wherein each of said specific binding partners are antibodies covalently attached to said signal layer, said analytes are antigens, and at least one of said test sections further comprises a conjugate of a detectable label and said antigen or an analog thereof specifically bound to said immobilized antibody.

5. The chemical sensor of claim 4 wherein said detectable label is a fluorescent label and said sensor further comprises:

a light source which irradiates said fluorescent label at a known excitation wavelength such that the fluorescent label emits light at a known emission wavelength to provide said optical signal and

a filter positioned between patterned signal layer and said two-dimensional light sensor array, wherein said filter removes transmitted light of the known excitation wavelength before said optical signal is detected by said two-dimensional light sensor array.

6. The chemical sensor of claim 5 wherein said filter is positioned between said two-dimensional lens array and said two-dimensional light sensor array.

7. The chemical sensor of claim 6 wherein said filter is sandwiched directly between said two-dimensional lens array and said two-dimensional light sensor array.

8. The chemical sensor of claim 1 wherein said two-dimensional light sensor array is a charge-coupled device array.

9. The chemical sensor of claim 1 wherein said two-dimensional array of graded index of refraction lenses is positioned between said patterned signal layer and said two-dimensional light sensor array.

10. A method of simultaneously detecting a plurality of analytes in a sample, comprising:

(a) providing a chemical sensor, said sensor comprising;

a patterned signal layer comprising a two-dimensional array of discrete, optically transparent test sections at known locations thereon, each of said test sections comprising an immobilized specific binding partner for one of said plurality of analytes, such that specific binding of said analyte to said immobilized specific binding partner generates an optical signal indicative of the presence of said analyte in said test section;

a two-dimensional light sensor array which detects said optical signal in a corresponding discrete detection section; and

a two-dimensional array of graded index of refraction lenses which focuses each said optical signal from each said test section into each said corresponding detection section;

(b) contacting said sample to said patterned signal layer; and

(c) detecting the presence or absence of said focused optical signal in each of said detection sections, whereby the presence of said focused optical signal in said detection section indicates the specific binding of the analyte in the test section and thereby indicates the presence of the analyte in the sample.

11. The method of claim 10 wherein said contacting step further comprises contacting said sample and said patterned signal layer with a labeled reagent comprising a fluorescent label conjugated to (i) either said analyte or an analog thereof which is specifically bound by said immobilized specific binding partner or (ii) a second specific binding partner which specifically binds to said analyte at a site which does not intefere with the specific binding between said analyte and said immobilized specific binding partner.

12. The method of claim 11, wherein said sensor further comprises a light source for irradiating said fluorescent label at a known excitation wavelength such that the fluorescent label emits light at a known emission wavelength to provide said optical signal and said method further comprises a step of irradiating said patterned signal layer with said excitation wavelength after said contacting step, thereby producing the focused optical signal detected in the detecting step.

13. The method of claim 12, wherein said sensor further comprises a filter positioned between patterned signal layer and said two-dimensional light sensor array, wherein said filter removes transmitted light of the known excitation wavelength before said optical signal is detected by said two-dimensional light sensor array and said detecting step further comprises removing said transmitted light of the known excitation wavelength before detecting said focused optical signal.

14. The method of claim 10 wherein said two-dimensional light sensor array is a charge-coupled device array.

15. A method of simultaneously detecting a plurality of analytes in a sample, comprising:

(a) providing a chemical sensor, said sensor comprising;

a patterned signal layer comprising a two-dimensional array of discrete, optically transparent test sections at known locations thereon, each of said test sections comprising an immobilized antibody which specifically binds to one of said plurality of analytes such that the specific binding generates an optical signal indicative of the presence of said analyte in said test section;

a two-dimensional light sensor array which detects said optical signal in a corresponding discrete detection section;

a two-dimensional array of graded index of refraction lenses which focuses each said optical signal from each said test section into each said corresponding detection section; and

,

a light source for irradiating a fluorescent label at a known excitation wavelength such that the fluorescent label emits light a known emission wavelength to provide said optical signal;

(b) contacting said sample, said patterned sign layer and a labeled reagent comprising said fluorescent label conjugated to (i) either said analyte or an analog thereof which is specifically bound by said immobilized antibody or (ii) a second antibody which specifically binds to said analyte at a site which does not interfere with the specific binding between said analyte and said immobilized antibody;

(c) irradiating said patterned signal layer with said excitation wavelength after said contacting step, thereby producing said focused optical signal; and

(d) detecting the presence or absence of said focused optical signal in each of said detection sections, whereby the presence of said focused optical signal indicates the specific binding of the analyte in the test section and thereby indicates the presence of the analyte in the sample.