Electrodes and metallo isoindole ringed compounds

• US 5,830,341 A
• Filed: 01/23/1996
• Issued: 11/03/1998
• Est. Priority Date: 01/23/1996
• Status: Expired due to Fees

• Current Assignee: Markas A.T. Gilmartin
• Original Assignee: Markas A.T. Gilmartin
• Inventors: Gilmartin, Markas A. T.
• Primary Examiner(s): Gorgos, Kathryn L.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US08/589,106
• Time in Patent Office: 1,015 Days
• Field of Search: 204/403, 205/777.5, 205/792, 205/778, 435/817
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The present invention provides for electrode assemblies and methods in which electron transfer between a redox reaction product and an electrically conductive electrode material is facilitated by a metallo macrocyclic compound, preferably a metallo isoindole ringed compound and more preferably a ferro isoindole ringed compound. The redox reaction is usually catalysized by a redox enzyme, such as an oxidase.

Claims

1. A hydrogen peroxide electrode assembly comprising:

a first hydrogen peroxide electrode comprising a metallo isoindole ringed compound dispersed in an electrode material, and

a first enzyme layer comprising a first enzyme in electrical contact with said first hydrogen peroxide electrode,

wherein said metallo isoindole ringed compound coordinates Fe, Ru, Rh, or Mn; and

wherein said first enzyme is attached to a first analyte binding moiety.

2. The hydrogen peroxide electrode assembly of claim 1, wherein said electrode material is a material selected from the group consisting of platinum, gold, silver and carbon.

3. The hydrogen peroxide electrode assembly of claim 2, wherein said electrode material further comprises electrically conductive carbon and said metallo isoindole ringed compound provides for sufficient electron transfer to permit detection of an electrical current.

4. The hydrogen peroxide electrode assembly of claim 3, wherein said metallo isoindole ringed compound is selected from the formula C₃₂ H₁₈ N₈ M;

where M is Fe or Mn and said first enzyme is a redox enzyme.

5. The hydrogen peroxide electrode assembly of claim 4, wherein said metallo isoindole ringed compound is of the formula C₃₂ H₁₈ N₈ Fe(II) and said redox enzyme is an oxidase.

6. The hydrogen peroxide electrode assembly of claim 5, further comprising:

a second hydrogen peroxide electrode comprising a metallo isoindole ringed compound dispersed in an electrode material, and

a second enzyme layer comprising a second enzyme and in electrical contact with said second detection electrode;

wherein said second hydrogen peroxide electrode is configured to separately measure hydrogen peroxide production in response to an additional analyte.

7. The hydrogen peroxide electrode assembly of claim 6, wherein said second enzyme is attached to a third analyte binding moiety that recognizes a second analyte and said third analyte binding moiety is different from said first analyte binding moiety, wherein said third analyte binding moiety permits detection of said additional analyte.

8. The hydrogen peroxide electrode assembly of claim 7, wherein said second enzyme layer comprises a matrix and a fourth analyte binding moiety attached to said matrix, wherein said fourth analyte binding moiety binds to said additional analyte and said fourth binding moiety is attached to said matrix to permit electrical contact with said second hydrogen peroxide electrode and to minimize electrical contact with said first hydrogen peroxide electrode.

9. The hydrogen peroxide electrode assembly of claim 8, further comprising four to ten hydrogen peroxide electrodes, wherein each said hydrogen peroxide electrode assembly separately measures hydrogen peroxide.

10. The hydrogen peroxide electrode assembly of claim 5, wherein said oxidase catalyses hydrogen peroxide production.

11. The hydrogen peroxide electrode assembly of claim 10, wherein said oxidase is an enzyme selected from the group consisting of 6-hydroxy-D-nicotine oxidase, alcohol oxidase, aldehyde oxidase, allyl-alcohol oxidase, amine oxidase, bilirubin oxidase, cholesterol oxidase, choline oxidase, cyclohexylamine oxidase, D-amino acid oxidase, D-aspartate oxidase, D-glutamate acid oxidase, dihydroorotate oxidase, dimethyl-glycine oxidase, ethanolamine oxidase, galactose oxidase, glucose oxidase, glycolate oxidase, glycollate oxidase, glyoxylate oxidase, hexose oxidase, L-amino acid oxidase, L-gluconolactone oxidase, L-glutamate acid oxidase, L-ricin α

-oxidase, L-sorbose oxidase, lactate oxidase, malate oxidase, N-methylamino acid oxidase, N⁶ -methylrycine oxidase, nitroethane oxidase, nucleoside oxidase, oxalate oxidase, putrecine oxidase, pyranose oxidase, pyridoxine 4-oxidase, pyruvate oxidase, rathosterol oxidase, sarcosine oxidase, sulphite oxidase, tyramine oxidase, urate oxidase, urease and xanthene oxidase, wherein said first enzyme provides sufficient activity to permit detection of hydrogen peroxide.

12. The hydrogen peroxide electrode assembly of claim 11, wherein said oxidase has glucose oxidase activity.

13. The hydrogen peroxide electrode assembly of claim 12, wherein said enzyme layer comprises a matrix and a second analyte binding moiety attached to said matrix.

14. The hydrogen peroxide electrode assembly of claim 13, wherein said first analyte binding moiety is an antibody and is covalently attached to said first enzyme.

15. The hydrogen peroxide electrode assembly of claim 12, wherein said oxidase is glucose oxidase and said glucose oxidase is directlv attached to said matrix.

16. The hydrogen peroxide electrode assembly of claim 15, wherein said matrix is selected from the group consisting of cellulose, polycarbonate, perfluorinated material, polystrole, polyurethane, polysulphone and silica.

17. The hydrogen peroxide electrode assembly of claim 16, wherein said matrix is screen printed.

18. The hydrogen peroxide electrode assembly of claim 17, further comprising a reference electrode for said hydrogen peroxide electrode.

19. The hydrogen peroxide electrode assembly of claim 18, wherein said enzyme layer comprises substances found in blood or urine.

20. The hydrogen peroxide electrode assembly of claim 19, wherein said first hydrogen peroxide electrode comprises a surface area that fits on a test card.

21. The hydrogen peroxide electrode assembly of claim 20, wherein said first hydrogen peroxide electrode and said enzyme layer are screen printed.

22. The hydrogen peroxide electrode assembly of claim 20, wherein said first hydrogen peroxide electrode comprises a working area with a width between 1 mm to 20 mm wide.

23. The hydrogen peroxide electrode assembly of claim 22, wherein said first hydrogen peroxide electrode is in a voltage field, wherein said voltage field has a voltage between -450 mV and -50 mV using an Ag/AgCl reference electrode, wherein said voltage field permits measurement of hydrogen peroxide reduction.

24. The hydrogen peroxide electrode assembly of claim 23, further comprising a scavenger layer comprising a second enzyme and said scavenger layer is located between said first hydrogen peroxide electrode and first enzyme layer.

25. The hydrogen peroxide electrode assembly of claim 24, wherein said second enzyme is L-ascorbic acid oxidase, wherein said L-ascorbic oxidase has sufficient activity to reduce the amount of dissolved oxygen in the presence of a substrate for said L-ascorbic acid oxidase.

26. The hydrogen peroxide electrode assembly of claim 15, further comprising a selectively permeable layer between said matrix and said electrode.

27. The hydrogen peroxide electrode assembly of claim 10, wherein said enzyme layer comprises a matrix and a second analyte binding moiety attached to said matrix, wherein said second analyte binding moiety recognizes said analyte.

28. The hydrogen peroxide electrode assembly of claim 27, wherein said first analyte binding moiety is an antibody and is covalently attached to said first enzyme.

29. The hydrogen peroxide electrode assembly of claim 28, wherein said oxidase produces a substrate for a second enzyme, wherein said second enzyme produces hydrogen peroxide.

30. The hydrogen peroxide electrode assembly of claim 29, wherein said oxidase has lactate oxidase activity and produces pyruvate and said second enzyme has pyruvate oxidase activity.

31. The hydrogen peroxide electrode assembly of claim 10, wherein said oxidase is attached to said matrix.

32. A method for electrically detecting the presence of an analyte comprising:

measuring an electrical current from a redox electrode comprising an electrode material and a ferro isoindole ringed compound in fluid communication with an oxidase,

wherein said oxidase catalyses a redox reaction that corresponds to the presence of an analyte and produces a product that either

     1) directly chemically oxidizes or reduces said ferro isoindole ringed compound or

     2) indirectly oxidizes or reduces said ferro isoindole ringed compound, and

wherein said oxidase is attached to a first analyte binding moiety.

33. The method of claim 32, further comprising:

applying a voltage field between said redox electrode and a reference electrode assembly.

34. The method of claim 33, wherein said applying step further comprises applying said voltage field between said redox electrode and said reference electrode assembly to permit electroreduction of said ferro isoindole ringed compound from C₃₂ H₁₈ N₈ Fe(III) to C₃₂ H₁₈ N₈ Fe(II).

35. The method of claim 34, further comprising

comparing said electrical current in the absence of said analyte and in the presence of said analyte.

36. The method of claim 35, further comprising:

quantitating the amount of said analyte in relation to the amount of said electrical current.

37. The method of claim 35, wherein said voltage field is between -50 and -450 mV.

38. The method of claim 37, wherein said product is hydrogen peroxide.

39. The method of claim 38, wherein said analyte is a substrate for said oxidase.

40. The method of claim 39, wherein said analyte is glucose.