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Methods of using oligomers containing modified pyrimidines

  • US 5,830,653 A
  • Filed: 06/07/1995
  • Issued: 11/03/1998
  • Est. Priority Date: 11/26/1991
  • Status: Expired due to Term
First Claim
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1. A method to evaluate a candidate antisense oligomer for its ability to inhibit gene expression, which method comprises:

  • microinjecting varying amounts of said candidate antisense oligomer into a host cell along with (a) a target vector for the expression of a gene containing a target sequence for said candidate antisense oligomer, and (b) a control vector for the expression of a control gene encoding a detectable protein, wherein said control gene does not contain said target sequence; and

    measuring expression of the target gene and the control gene;

    wherein increasing inhibition of the target gene expression, but not of the control gene expression, as the amount of said candidate antisense oligomer increases, indicates the ability of said candidate antisense oligomer to inhibit gene expression.

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