Neural thread protein gene expression and detection of Alzheimer's disease
First Claim
1. A method of diagnosing the presence of Alzheimer'"'"'s Disease in a human subject suspected of having Alzheimer'"'"'s Disease which comprises:
- (a) incubating a biological sample from said subject which is suspected of containing neural thread protein (NTP) having a molecular weight observed by SDS-PAGE of about 8 kDa, about 14 kDa, about 17 kDa, about 21 kDa, about 26 kDa or about 42 kDa, in the presence of at least one antibody that specifically binds said NTP and does not cross-react with pancreatic thread protein; and
(b) detecting said antibody which is bound in said sample, wherein detection of elevated levels of NTP indicates that said subject has Alzheimer'"'"'s Disease.
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Abstract
The present invention is directed to recombinant hosts expressing novel proteins associated with Alzheimer'"'"'s Disease, neuroectodermal tumors, malignant astrocytomas, and glioblastomas. This invention is specifically directed to the recombinant hosts and vectors which contain the genes coding for the neuronal thread proteins. This invention is also directed to substantially pure neural thread protein, immunodiagnostic and molecular diagnostic methods to detect the presence of neural thread proteins, and the use of nucleic acid sequences which code for neural thread proteins in gene therapy.
80 Citations
25 Claims
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1. A method of diagnosing the presence of Alzheimer'"'"'s Disease in a human subject suspected of having Alzheimer'"'"'s Disease which comprises:
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(a) incubating a biological sample from said subject which is suspected of containing neural thread protein (NTP) having a molecular weight observed by SDS-PAGE of about 8 kDa, about 14 kDa, about 17 kDa, about 21 kDa, about 26 kDa or about 42 kDa, in the presence of at least one antibody that specifically binds said NTP and does not cross-react with pancreatic thread protein; and (b) detecting said antibody which is bound in said sample, wherein detection of elevated levels of NTP indicates that said subject has Alzheimer'"'"'s Disease. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for detecting the presence of Neural Thread Protein (NTP) having a molecular weight observed by SDS PAGE of about 8 kDa, about 14 kDa, about 17 kDa, about 21 kDa, about 26 kDa or about 42 kDa, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69257, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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19. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69258, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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20. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69259, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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21. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the DH1 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69260, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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22. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69261, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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23. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the DH1 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69263, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds to said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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24. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69264, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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25. A method for detecting the presence of Neural Thread Protein (NTP) identical to that coded for by the DNA molecule present in the DH5 E. coli cells that are on deposit at the American Type Culture Collection, Manassas, Va., under accession number 69265, in a human subject, said method comprising:
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(a) contacting a biological sample from said human subject that is suspected of containing said NTP with at least one antibody that specifically binds said protein and does not cross-react with pancreatic thread protein; and (b) detecting any of said antibody bound to said protein.
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Specification