Bioassay of selenium
First Claim
1. A plasmid carrying a selenium-specifying DNA sequence of the Escherichia coli (E. coli) formate dehydrogenase (fdhF) gene of the -9 to +47 nucleotide bases or -1 to +47 nucleotide bases encoding the TGA region upstream of the E. coli lac'"'"'Z gene which permits the incorporation of selenocysteine into β
- -galactosidase.
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Accused Products
Abstract
The invention relates to a plasmid carrying a selenium-specifying DNA seqce of the E. coli fdhF gene upstream of the E. coli lac'"'"'Z gene which permits the incorporation of selenocystein into β-galactosidase. Particular plasmids according to the invention are pRM2, deposited at the ATCC under No. 75594 and pRM4, deposited at the ATCC under No. 75595. The invention also relates to microorganisms transformed with a plasmid according to the invention having selenium dependent β-galactosidase activity. The invention provides a method for the quantitative determine of selenium in selenium derivatives in a biological sample comprising incubating microorganisms according to the invention in a suitable medium also containing said sample and measuring the level of β-galactosidase activity. The biological sample may be, e.g. a blood sample or a food sample.
5 Citations
29 Claims
- 1. A plasmid carrying a selenium-specifying DNA sequence of the Escherichia coli (E. coli) formate dehydrogenase (fdhF) gene of the -9 to +47 nucleotide bases or -1 to +47 nucleotide bases encoding the TGA region upstream of the E. coli lac'"'"'Z gene which permits the incorporation of selenocysteine into β
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5. Plasmid pRM2 deposited at the ATCC under No. 75594.
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6. Plasmid pRM4 deposited at the ATCC under No. 75595.
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9. E. coli transformed with plasmid pRM2.
- 10. E. coli transformed with plasmid pRM4.
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28. A plasmid carrying a selenium-specifying DNA sequence of the Escherichia coli (E. coli) formate dehydrogenase (fdhF) gene of the -9 to +47 nucleotide bases encoding the TGA region upstream of a E. coli lac'"'"'Z gene derivative which permits the incorporation of selenocysteine into β
- -galactosidase.
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29. A plasmid carrying a selenium-specifying DNA sequence of the Escherichia coli (E. coli) formate dehydrogenase (fdhF) gene of the -1 to +47 nucleotide bases encoding the TGA region upstream of a E. coli lac'"'"'Z gene derivative which permits the incorporation of selenocysteine into β
- -galactosidase.
Specification