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Selective template deletion method

  • US 5,830,712 A
  • Filed: 02/06/1996
  • Issued: 11/03/1998
  • Est. Priority Date: 02/06/1996
  • Status: Expired due to Fees
First Claim
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1. A method of reducing cloning interference by undesirable nucleic acids in a mRNA sample, comprising:

  • providing a mRNA sample which comprises a nucleic acid family comprising desirable nucleic acids which are to be cloned in a desirable nucleic acids cloning process and undesirable nucleic acids which are capable of interfering in the desirable nucleic acids cloning process;

    combining the mRNA sample with a blocker capable of selectively associating with the undesirable nucleic acids and forming a hybrid between the blocker and the undesirable nucleic acids such that the hybrid is incapable of participating in the desirable nucleic acids cloning process; and

    cleaving the hybrid formed in said combining step.

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