Selective template deletion method
First Claim
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1. A method of reducing cloning interference by undesirable nucleic acids in a mRNA sample, comprising:
- providing a mRNA sample which comprises a nucleic acid family comprising desirable nucleic acids which are to be cloned in a desirable nucleic acids cloning process and undesirable nucleic acids which are capable of interfering in the desirable nucleic acids cloning process;
combining the mRNA sample with a blocker capable of selectively associating with the undesirable nucleic acids and forming a hybrid between the blocker and the undesirable nucleic acids such that the hybrid is incapable of participating in the desirable nucleic acids cloning process; and
cleaving the hybrid formed in said combining step.
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Abstract
A novel method for inactivating undesirable members in a nucleic acid sample which comprises undesirable and desirable members of a nucleic acid family. The method includes the step of adding a blocker to the sample which selectively associates with the undesirable members such that they are unable to participate in any further processing of the sample. The method advantageously provides a means whereby desirable members of the nucleic acid family can be processed, i.e. identified, amplified and/or isolated, without interference from undesirable sequence-related members.
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Citations
3 Claims
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1. A method of reducing cloning interference by undesirable nucleic acids in a mRNA sample, comprising:
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providing a mRNA sample which comprises a nucleic acid family comprising desirable nucleic acids which are to be cloned in a desirable nucleic acids cloning process and undesirable nucleic acids which are capable of interfering in the desirable nucleic acids cloning process; combining the mRNA sample with a blocker capable of selectively associating with the undesirable nucleic acids and forming a hybrid between the blocker and the undesirable nucleic acids such that the hybrid is incapable of participating in the desirable nucleic acids cloning process; and cleaving the hybrid formed in said combining step. - View Dependent Claims (2, 3)
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Specification
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Current AssigneeNPS Pharmaceuticals Incorporated (Shire plc)
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Original AssigneeAllelix Biopharmaceuticals, Inc. (NPS Allelix Corp.)
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InventorsRampersad, Vikarna, Zastawny, Roman, Kamboj, Rajender
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Primary Examiner(s)Jones, W. Gary
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Assistant Examiner(s)FREDMAN, JEFFREY NORMAN
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Application NumberUS08/597,249Time in Patent Office1,001 DaysField of Search435/6, 435/91.1, 435/91.2, 536/24.3, 536/24.31, 536/24.32, 536/24.33, 536/24.5, 536/22.1, 536/23.1US Class Current435/91.1CPC Class CodesC12Q 1/6848 characterised by the means ...C12Q 2525/186 incorporating a non-extenda...C12Q 2537/161 A competitive reaction step
