Sample collection device

• US 5,833,630 A
• Filed: 01/30/1997
• Issued: 11/10/1998
• Est. Priority Date: 11/03/1994
• Status: Expired due to Fees

• Current Assignee: Labor Laborgerate + Analysensysteme Vertriebsgesellschaft Mbh
• Original Assignee: Bernd Kloth
• Inventors: Kloth, Bernd
• Primary Examiner(s): HINDENBURG, MAX F
• Application Number:
  US08/792,469
• Time in Patent Office: 649 Days
• Field of Search: 600/573, 600/576, 600/583, 600/584, 606/181, 604/36, 604/37, 604/51, 604/181
• US Class Current: 604/509
• CPC Class Codes: A61B 5/150022 for capillary blood or interstitial fluid
• CPC Class Codes: A61B 5/150343 Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes sampling from within body cavities A61B5/150015, A61B5/150045
• CPC Class Codes: A61B 5/150755 Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
• CPC Class Codes: G01N 33/86 involving blood coagulating time or factors, or their receptors

Abstract

In order to provide a sample collection device (100) for the collection of capillary blood directly from the patient or for taking a sample from a liquid with the aid of a capillary (16) so that it is made possible to perform analyses of the sample free from volume errors within the sample area and free from contamination, it is proposed that the capillary (16), for the absorption of sample liquid to be analyzed, be connectable with the aid of a mounting means (100) with a cuvet (10) possibly containing a reagent liquid.

Claims

1. A sample collection device for the collection and analysis of the coagulation time of capillary blood taken directly from a patient with the aid of a capillary, comprising a unitary combination of a) through f):

a) a capillary for the capillary attraction into the capillary of a predetermined quantity of blood to be analyzed directly from the patient;

b) a cuvet;

c) a reagent liquid contained within said cuvet;

d) an agitator member contained within said cuvet and surrounded by said reagent liquid;

e) a means for mounting said capillary, said mounting means comprising;

a capillary holder, said capillary holder retaining said capillary in a position disposed on said cuvet and extending into said cuvet with a plug-like section of said capillary holder, said capillary holder possessing an extension on the side facing away from said cuvet in the center of said capillary holder; and

f) a protective and pressure cap longitudinally displacable on and in pressure sealable relationship with said extension of said capillary holder, the downward movement of the pressure cap creating a gas pressure in the capillary which forces blood in the capillary into the reagent;

g) a measuring canal for the cuvet; and

h) an analyzer having an internal memory, the analysis process of which is activated by entry of blood into the cuvet; and

i) recording the coagulation time of the blood in the internal memory of the analyzer.

2. A sample collection device according to claim 1 wherein a through bore extends through the capillary holder:

said capillary is mounted in said capillary holder so as to be longitudinally displacable into the reagent by the downward movement of the pressure cap.

3. A sample collection device according to claim 1, wherein said cuvet is sealed with the aid of said capillary holder.

4. A sample collection device according to claim 1, wherein said protective and pressure cap is constructed so as to be placeable into a first position engaging only partially over said extension and into a second position in which it can be slid forward so as to engage completely over said extension.

5. A sample collection device according to claim 1, wherein withing a contact area of said plug on said cuvet or within only a contact area of said extension under the pressure cap before the cap is depressed, or both, grooves are constructed in the direction toward said cuvet which permit a pressure compensation between the interior of said cuvet and the external environment when the pressure cap is depressed.

6. A sample collection device according to claim 1, wherein said circumferential rim of said capillary holder is moved downwardly in the direction of said cuvet on the exterior of said cuvet.

7. A sample collection device according to claim 1, wherein an aperture of said capillary on the side facing away from said cuvet can be acted upon by a compressed gas with the aid of a means which forms part of or is a supplementary part of said sample collection device, in such a way that, between said aperture on the side facing away from said cuvet and an aperture on the side facing toward said cuvet of said capillary, a defined pressure difference exists, said defined pressure difference is regulated in such a way that, by the evacuation of a sample liquid into said cuvet, the pressure difference is compensated by the pressurization of a small quantity of gas that escapes.

8. A sample collection device according claim 7, wherein said small quantity of gas corresponds to the volume of said capillary, preferably 1/10 through 1/2 of the volume.

9. A sample collection device according to claim 7, wherein said small quantity of gas is air.

10. A sample collection device according to claim 1, wherein the volume in said protective and pressure cap is regulated in such a way that, when said protective and pressure cap is placed upon said extension and is subsequently pressed down completely, a sample liquid is urged out from said capillary.

11. A sample collection device according to claim 1, wherein said cuvet, when separated from said capillary holder, is sealed by means of an evaporation-proof foil or a detachable cuvet cap.

12. A sample collection device according to claim 11, wherein said cuvet cap can be employed as a pressure cap and is constructed so as to be placeable into a first position in which it engages only partially over said extension and into a second position, in which it engages completely over said extension.

13. A sample collection device according to claim 12, wherein an internal volume in said cuvet cap constructed in the form of a pressure cap is regulated to urge a sample liquid from said capillary when said cuvet cap is placed on said extension and when subsequently depressed completely.

14. A sample collection device according to claim 1, wherein an anti-coagulant, such as a citrate buffer, is contained in a reagent liquid in said cuvet.

15. A sample collection device according to claim 1, wherein said capillary is an end-to-end capillary.

16. A sample collection device according to claim 1, wherein a through bore in said capillary holder is shorter than the length of said capillary.

17. A sample collection device according to claim 16, wherein said through bore in said capillary holder is 20% to 30% shorter than the length of said capillary.

18. A sample collection device for the collection of an accurately measured amount and analysis of capillary blood taken directly from a patient or from a liquid with the aid of a capillary, comprising a unitary combination of a) through e):

a) a capillary for the absorbtion by capillary attraction of an accurately measured amount of sample liquid to be analyzed,

b) a cuvet;

c) a means for mounting said capillary on said cuvet; and

d) a pressure cap means for creating a pressure at one end of the capillary which transfers the blood in the capillary into the cuvet; and

e) a means for analyzing said sample liquid within said cuvet without removing said capillary and mounting means.

19. A unitary sample collection device comprising the assembly of a) through e):

a) a cuvette wherein the pressure in the cuvet is atmospheric;

b) a plug in the cuvette;

c) a through bore through the plug;

d) a longitudinally displacable pressure cap for the plug having a travel path over an end of the plug to create a pressure; and

e) a capillary in the through bore.

20. The sample collection device of claim 19 wherein contents of the cuvet from an end of the capillary in the cuvet to a terminal end of the cuvet opposed to the plug consist essentially of air, reagent and a stirring member, free of a restraining membrane.