Production of heterologous peptides
First Claim
1. A construct including at least the regulated promoter sequence of the gene encoding the protein glucoamylase of Neurospora crassa and further including at least one restriction site whereby a coding sequence for a heterologous peptide can be introduced into the gene so a heterologous peptide is expressed under the control of said promoter sequence.
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Abstract
The invention relates to a method and recombinant means for engineering the production of heterologous peptides in filamentous fungus. The invention involves the genetic manipulation of the glucoamylase gene in order to provide a restriction site in same so that the promoter sequence of the glucoamylase gene can be coupled to a sequence encoding a heterologous peptide whereby the production of the peptide will be under the control of the promoter sequence of the glucoamylase gene.
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Citations
26 Claims
- 1. A construct including at least the regulated promoter sequence of the gene encoding the protein glucoamylase of Neurospora crassa and further including at least one restriction site whereby a coding sequence for a heterologous peptide can be introduced into the gene so a heterologous peptide is expressed under the control of said promoter sequence.
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26. A primer comprising at least one of the sequence structures shown in FIG. 4, SEQ ID NO:
- 8, SEQ ID NO;
9, SEQ ID NO;
10, SEQ ID NO;
11, SEQ ID NO;
12 or SEQ ID NO;
13.
- 8, SEQ ID NO;
Specification