Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
First Claim
1. A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of:
- (a) providing a plurality of wide-band emission filters;
(b) for each of the pixels, recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters;
(c) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
1 Assignment
0 Petitions
Accused Products
Abstract
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
83 Citations
49 Claims
-
1. A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of:
-
(a) providing a plurality of wide-band emission filters; (b) for each of the pixels, recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
-
13. A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of:
-
(a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of said wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of said plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
-
-
31. An apparatus for classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the apparatus comprising:
-
(a) a light source; (b) a plurality of pairs of wide-band excitation filters and wide-band emission filters; (c) a control device for; (i) selecting a pair of said plurality of pairs; (ii) exciting fluorophores of each of the pixels with light originating from said light source filtered through one of said wide-band excitation filters; and (iii) repeating steps (i)-(ii) for all of said plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) a light intensity recording device for recording emitted light intensity as retrieved after passing through said emission filters; and (e) a computing device including an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
-
-
47. A method of determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of:
-
(a) providing a plurality of wide-band emission filters; (b) recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters; (c) using an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
-
-
48. A method of determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of:
-
(a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of the pixel with light filtered through one of said wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of said plurality of pairs of filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; and (d) using an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
-
-
49. An apparatus far determining the amount of a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores associated with a pixel, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the apparatus comprising:
-
(a) a light source; (b) a plurality of pairs of wide-band excitation filters and wide-band emission filters; (c) a control device for; (i) selecting a pair of said plurality of pairs; (ii) exciting fluorophores of the pixel with light originating from said light source filtered through one of said wide-band excitation filters; and (iii) repeating steps (i)-(ii) for all of said plurality of pairs of filters, such that the pixel is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of said plurality of pairs of filters; (d) a light recording device for recording emitted light intensity as retrieved after passing through said emission filters; and (e) a computing device including an algorithm for evaluating the amount of each of the plurality of fluorophores associated with the pixel.
-
Specification