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Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore

  • US 5,834,203 A
  • Filed: 08/25/1997
  • Issued: 11/10/1998
  • Est. Priority Date: 08/25/1997
  • Status: Expired due to Term
First Claim
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1. A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing emission spectrum and specifying emission peak, the method comprising the steps of:

  • (a) providing a plurality of wide-band emission filters;

    (b) for each of the pixels, recording emitted light intensity as retrieved after passing through each one of said plurality of emission filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of plurality of wide-band emission filters;

    (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores associated with each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.

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