Biochemical sensor

• US 5,834,218 A
• Filed: 10/11/1996
• Issued: 11/10/1998
• Est. Priority Date: 02/17/1995
• Status: Expired due to Fees

• Current Assignee: Thomson - Csf
• Original Assignee: Thomson-CSF
• Inventors: Gremillet, Jacques
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/722,018
• Time in Patent Office: 760 Days
• Field of Search: 385/12, 385/129, 385/130, 422/55, 422/57, 422/82.05, 422/82.08, 422/82.09, 422/82.11, 435/7.31, 435/287.1, 435/287.2, 435/288.7, 435/808, 435/973, 436/518, 436/524, 436/527, 436/164, 436/165, 436/172, 436/805, 436/809
• US Class Current: 435/7.31
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

The invention relates to a biochemical sensor including a substrate on which yeast cells are deposited which are capable of capturing one type of molecules (M) and of producing a chemical entity (P) at the outcome of the capture; it also includes means for detecting the entity (P). The yeast cells employed are yeast cells obtained by genetic manipulation, in which the capture sites have been adapted to the type of molecules (M).

Claims

1. Biochemical sensor characterized in that it comprises:

a substrate on which yeast cells are deposited which are capable of capturing one type of molecules (M) and of producing a chemical entity (P) at the outcome of this capture;

means for detecting the chemical entity (P) produced.

2. Biochemical sensor according to claim 1, characterized in that the yeast cells are yeast cells obtained by mutagenesis, wherein the mutagenesis causes the yeast cells to contain capture sites capable of capturing the type of molecules (M).

3. Biochemical sensor according to claim 1, characterized in that the yeast cells are yeast cells obtained by genetic manipulation of the genotype of said cells so as to adapt the capture sites of said cells to one type of molecules (M).

4. Biochemical sensor according to claim 1, wherein the substrate is a gel filled with sugar and inorganic salts.

5. Biochemical sensor according to claim 1, wherein the substrate is filled with yeast cells, and the substrate can be activated by moistening.

6. Biochemical sensor according to claim 1, wherein the entity (P) is a protein.

7. Biochemical sensor according to claim 1, wherein the biochemical sensor further comprises a physical means of detection which are optical means for measuring optical density, change in light polarization or fluorescence.

8. Biochemical sensor characterized in that it comprises a mosaic of elementary biochemical sensors according to claim 1, each elementary sensor being capable of detecting one type of molecule (Mi), the combination of the responses supplied by the means of detections (m_i) of each sensor making it possible to identify molecules present in a medium.