Assay for cardiac troponin I
First Claim
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1. An assay for quantitation of cardiac troponin I in a human biological fluid, comprising:
- a. incubating sequentially or simultaneously;
i) a sample of the human biological fluid;
ii) antibody or antibody fragment specific for all or an antibody-reactive portion of the cardiac troponin I peptide ##STR5## iii) troponin C or a troponin I-binding fragment thereof, under conditions which allow the formation of a ternary complex of troponin I in the sample, the antibody and the troponin C;
b. separating the ternary complex from the sample; and
c. detecting the formation of the ternary complex as indicative of the amount of cardiac troponin I in the human biological fluid.
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Abstract
An assay, and kit, for the quantitation of cardiac troponin I in human biological fluid is provided. The assay involves a sandwich assay employing an antibody specific for cardiac troponin I as one binding partner for cardiac troponin I and troponin C as the other binding partner for cardiac troponin I.
43 Citations
27 Claims
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1. An assay for quantitation of cardiac troponin I in a human biological fluid, comprising:
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a. incubating sequentially or simultaneously; i) a sample of the human biological fluid; ii) antibody or antibody fragment specific for all or an antibody-reactive portion of the cardiac troponin I peptide ##STR5## iii) troponin C or a troponin I-binding fragment thereof, under conditions which allow the formation of a ternary complex of troponin I in the sample, the antibody and the troponin C; b. separating the ternary complex from the sample; and c. detecting the formation of the ternary complex as indicative of the amount of cardiac troponin I in the human biological fluid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A kit containing reagents for performing an assay for human cardiac troponin I, comprising:
a. antibody or antibody fragment specific for all or an antibody-reactive portion of the cardiac troponin I peptide ##STR6## b. troponin C or a troponin I-binding fragment thereof. - View Dependent Claims (14, 15, 16, 17)
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18. A solid phase immunoassay for quantitation of troponin I in a human biological fluid, comprising:
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a. incubating sequentially or simultaneously; i) a sample of the human biological fluid; ii) a solid phase immunoadsorbent comprising immobilized polyclonal antibody or antibody fragment specific for all or an antibody-reactive portion of the cardiac troponin peptide ##STR7## iii) labeled troponin C or a troponin I-binding fragment thereof, under conditions sufficient for troponin I in the sample to complex with the immobilized antibody and the labeled troponin C; b. separating the solid phase immunoadsorbent from the sample; and c. detecting the amount of labeled troponin C or fragment thereof, bound to the solid phase immunoadsorbent or the amount of unbound labeled troponin C or fragment thereof, as indicative of the amount of cardiac troponin I in the human biological fluid. - View Dependent Claims (19, 20, 21, 22, 23)
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24. A kit of reagents for performing a solid phase immunoassay for human troponin I, comprising:
a. a solid phase immunoadsorbent containing polyclonal antibody or antibody fragment specific for all or an immunoreactive portion of the cardiac troponin I peptide ##STR8## b. labeled troponin C or a troponin I-binding fragment thereof. - View Dependent Claims (25, 26, 27)
Specification