Pharmaceutical composition containing sialic acid derivatives

• US 5,834,423 A
• Filed: 03/15/1996
• Issued: 11/10/1998
• Est. Priority Date: 07/15/1994
• Status: Expired due to Term

• Current Assignee: Taiyo Kagaku Company Limited
• Original Assignee: Taiyo Kagaku Company Limited
• Inventors: Koketsu, Mamoru, Kawanami, Hiroshi, Nitoda, Teruhiko, Enoki, Yuko, Nishizono, Masakazu, Juneja, Lekh Raj
• Primary Examiner(s): Kight, John
• Assistant Examiner(s): SEAMAN, D MARGARET M
• Application Number:
  US08/617,821
• Time in Patent Office: 970 Days
• Field of Search: 536/2, 536/17.2, 536/123, 536/123.1, 514/7, 514/8, 514/22, 514/55, 514/56
• US Class Current: 514/2.4
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/7012 Compounds having a free or esterified carboxyl group attached, directly or through a carbon chain, to a carbon atom of the saccharide radical, e.g. glucuronic acid, neuraminic acid gluconic acid A61K31/191; ascorbic acid A61K31/375
• CPC Class Codes: A61K 36/736 Prunus, e.g. plum, cherry, peach, apricot or almond
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 1/12 Antidiarrhoeals
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 37/06 Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 37/08 Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: C07H 3/06 Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages

Abstract

The present invention is a pharmaceutical composition used as an antiviral agent, a diarrheal remedy, an antiulcer agent, an anti-inflammatory agent, an anti-allergic agent and an agent for promoting the proliferation of Bifidobacteria, characterized by containing a sialic acid derivative as an active ingredient. The method for producing a sialic acid derivative of the present invention is characterized in that an almond or apricot seed is added to an avian egg yolk, by which the desired product can easily be obtained in a high yield.

Claims

1. A pharmaceutical composition characterized by containing as an active ingredient a sialic acid derivative represented by the general formula (1) or (2) below:

##STR15## wherein R¹ represents a glycopeptide residue; and

X¹ and X², which may be identical or different, represent NeuAc(α

2,6)Gal(β

1,4)GlcNAc(β

1,2)-, Gal(β

1,4)GlcNAc(β

1,2)-, or GlcNAc(β

1,2)-, provided that at least one of X¹ and X² represents NeuAc(α

2,6)Gal(β

1,4)Glc NAc(β

1,2)-;

##STR16## wherein R² and R³, which may be identical or different, represent a hydrogen atom or a linear, branched or cyclic, saturated or unsaturated acyl group having 1 to 30 carbon atoms;

n is an integer of 1 to 20; and

X³ represents a sialic acid derivative residue or a sialyloligosaccharide derivative residue represented by the general formula (3) below;

##STR17## wherein X⁴ and X⁵, which may be identical or different, represent NeuAc(α

2,6)Gal(β

1,4)GlcNAc(β

1,2)-, Gal(β

1,4)GlcNAc(β

1,2)- or GlcNAc(β

1,2)-, provided that at least one of X⁴ and X⁵ represents NeuAc(α

2,6)Gal(β

1,4)Glc NAc(β

1,2)-; and

Y represents a sugar residue.

9. A method of treating illness in a patient comprising administering an effective amount of the pharmaceutical composition of claim 1 to a patient in need thereof, wherein said illness is diarrhea, allergy or inflammation.

10. The method according to claim 9, wherein said illness is caused by a microorganism.

11. The method according to claim 10, wherein said illness is caused by a virus.

12. The method according to claim 11, wherein said illness is diarrhea.

13. The method according to claim 10, wherein said illness is caused by a bacteria.

14. The method according to claim 13, wherein said illness is diarrhea.

15. A method of treating illness in a patient comprising administering an effective amount of the pharmaceutical composition of claim 1 to a patient in need thereof, wherein said pharmaceutical composition promotes the proliferation of Bifidobacteria.

16. The method according to claim 9, wherein said illness is allergy.

17. The method according to claim 9, wherein said illness is inflammation.

18. The method according to claim 9, wherein the effective amount is 0.001 to 1000 mg per kg body weight.

19. The method according to claim 10, wherein the effective amount is 0.001 to 10 mg per kg body weight.

20. The method according to claim 11, wherein the effective amount is 0.001 to 0.3 mg per kg body weight.

21. The method according to claim 9, wherein the illness is diarrhea and the effective amount is 0.5 to 10 mg per kg body weight.

22. The method according to claim 13, wherein the effective amount is 0.001 to 2 mg per kg body weight.

23. A method of treating an ulcer caused by in a patient comprising administering an effective amount of the pharmaceutical composition of claim 1 to a patient in need thereof.

24. The method according to claim 23, wherein the effective amount is 0.001 to 2 mg per kg body weight.

25. The method according to claim 16, wherein the effective amount is 100 to 1000 mg per kg body weight.

26. The method according to claim 9, wherein the illness is allergy and the effective amount is 0.001 to 0.3 mg per kg body weight.

27. The method according to claim 9, wherein the illness is inflammation and the effective amount is 0.001 to 0.1

2. A method for producing a sialic acid derivative represented by the following general formula (4) ##STR18## wherein R⁴ represents a sugar residue;

X¹ and X², which may be identical or different, represent NeuAc(α

2,6)Gal(β

1,4)GlcNAc(β

1,2)-, Gal(β

1,4)GlcNAc(β

1,2)- or GlcNAc(β

1,2)-, provided that at least one of X¹ and X² represents NeuAc(α

2,6)Gal(β

1,4)GlcNAc(β

1,2)- comprising the steps of;

(a) adding water or brine selected from a potassium salt, a sodium salt and a salt capable of buffering over the range of pH 5 to 10 to avian egg yolk,

(b) stirring and filtering the mixture obtained in step (a),

(c) adding almond or apricot seed to the filtrate obtained in step (b),

(d) stirring the mixture obtained in step (c),

(e) subjecting the mixture obtained in step (d) to an anion exchange resin and carrying out elution, and

(f) desalting the eluate obtained in step (e).

3. The method according to claim 2, wherein the almond is delipidated.

4. The method according to claim 2, wherein the apricot seed is delipidated.

5. The method according to claim 2, wherein said avian egg yolk is delipidated.

6. The method according to claim 2, wherein the stirring in step (b) is carried out at 40°

to 80°

C.

7. The method according to claim 2, wherein the stirring in step (d) is carried out at pH 5.0 to 5.5 and 30°

to 45°

C.

8. The method according to claim 2, wherein the elution in step (e) is carried out using an NaCl solution or a sodium acetate solution.