Amines and methods of making and using the same

• US 5,834,607 A
• Filed: 12/22/1994
• Issued: 11/10/1998
• Est. Priority Date: 07/27/1990
• Status: Expired due to Term

• Current Assignee: ISIS Pharmaceuticals Incorporated
• Original Assignee: ISIS Pharmaceuticals Incorporated
• Inventors: Manoharan, Muthiah, Cook, P. Dan
• Primary Examiner(s): Elliott, George C.
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US08/361,858
• Time in Patent Office: 1,419 Days
• Field of Search: 435/6, 435/860, 436/501, 514/44, 536/22.1, 536/23.1, 536/24.1, 536/24.3-24.33, 935/77, 935/78
• US Class Current: 536/22.1
• CPC Class Codes: A61K 47/59 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
• CPC Class Codes: C07D 405/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: C07D 405/14 containing three or more hetero rings
• CPC Class Codes: C07F 7/1804 Compounds having Si-O-C linkages Si-O-acyl linkages C07F7/1896
• CPC Class Codes: C07H 19/04 Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
• CPC Class Codes: C07H 19/06 Pyrimidine radicals
• CPC Class Codes: C07H 19/10 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 19/16 Purine radicals
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

Novel amine compounds are provided by the present invention. Methods of preparing and using said novel amine compounds are also provided.

Claims

1. A compound having the structure:

##STR14## wherein R₄ is an oligonucleotide and M is a pendent group having a polyamine species attached thereto.

2. The compound of claim 1 wherein M is R₇ S or R₇ NH wherein R₇ is a polyamine species.

3. The compound of claim 2 wherein the polyamine species comprises at least one nitrogen atom having a free electron pair.

4. The compound of claim 1 further including one or more of reporter groups, alkylating agents, intercalating agents, cell receptor binding molecules, steroids, peptides, crown amines, porphyrins, or cross-linking agents attached to at least one of the nitrogen atoms of said polyamine.

5. The compound of claim 1 in a pharmaceutically acceptable carrier.