Multi-wavelength optical microscope
First Claim
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1. A multi-wavelength optical microscope comprising:
- a plurality of light sources; and
wavelength varying means for independently varying the wavelength of individual ones of said plurality of light sources such that at least one of said plurality of light sources irradiates a light beam having a wavelength capable of causing the transition of a sample molecule from a ground state to an excited state and, within a lifetime of the excited state, another one of said plurality of light sources irradiates a light beam having a wavelength capable of causing the transition of the sample molecule from the excited state to a higher-order excited state to obtain one of a) an absorption image when the ground state transits to the higher-order excited state via the excited state, and b) a light emitting image when the higher-order excited state decays to the ground state via the excited state.
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Abstract
A high-accuracy multi-wavelength optical microscope for providing a satisfactory contrast of image and much information about a sample. The microscope includes a plurality of light sources, a wavelength varietor independently varying the wavelength of the individual light sources, and polarization plane rotators on the optical path for each light source.
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Citations
6 Claims
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1. A multi-wavelength optical microscope comprising:
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a plurality of light sources; and wavelength varying means for independently varying the wavelength of individual ones of said plurality of light sources such that at least one of said plurality of light sources irradiates a light beam having a wavelength capable of causing the transition of a sample molecule from a ground state to an excited state and, within a lifetime of the excited state, another one of said plurality of light sources irradiates a light beam having a wavelength capable of causing the transition of the sample molecule from the excited state to a higher-order excited state to obtain one of a) an absorption image when the ground state transits to the higher-order excited state via the excited state, and b) a light emitting image when the higher-order excited state decays to the ground state via the excited state. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification
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Current AssigneeResearch Development Corporation Of Japan (Japan Science And Technology Agency), Olympus Optical Company Ltd. (Olympus Corporation)
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Original AssigneeResearch Development Corporation Of Japan (Japan Science And Technology Agency), Olympus Optical Company Ltd. (Olympus Corporation)
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InventorsFujii, Masaaki, Iketaki, Yoshinori
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Primary Examiner(s)Henry, Jon W.
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Assistant Examiner(s)Robinson, Mark A.
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Application NumberUS08/580,196Time in Patent Office1,048 DaysField of Search359/368, 359/363, 359/371, 359/386, 359/352, 359/355, 250/458.1US Class Current359/352CPC Class CodesG02B 21/06 Means for illuminating spec...
