Process for regenerating bone and cartilage

• US 5,837,235 A
• Filed: 05/09/1996
• Issued: 11/17/1998
• Est. Priority Date: 07/08/1994
• Status: Expired due to Term

• Current Assignee: DePuy Orthopaedics Incorporated (Johnson & Johnson), Ethicon, LLC (Johnson & Johnson)
• Original Assignee: Sulzer Medizinaltechnik AG
• Inventors: Mueller, Werner, Thaler, Thomas
• Primary Examiner(s): Naff, David M.
• Application Number:
  US08/602,833
• Time in Patent Office: 922 Days
• Field of Search: 424/93.7, 424/520, 435/174, 435/176, 435/177, 435/180, 435/182, 435/240.2, 435/240.23, 435/395, 435/396, 435/402
• US Class Current: 424/93.7
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61K 35/37 Digestive system
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/36 containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix isolated natural compounds, e.g. collagen A61L27/24
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3847 Bones
• CPC Class Codes: A61L 27/3852 Cartilage, e.g. meniscus

Abstract

Bone and cartilage are regenerated in a patient by a process of removing fatty tissue such as omentum tissue from a patient, comminuting the tissue to form small tissue particles, suspending the particles in a liquid to form a suspension, depositing the suspension on a solid carrier to prepare a solid implanting material, implanting the implanting material in a patient in an environment favoring bone or cartilage formation, and regenerating bone or cartilage in the patient. The carrier can be demineralized bone, collagen, mineral material or synthetic polymer material in pulverulent, textile, porous particle or monolith form. A cell adhesion agent may be applied to the carrier or added to the suspension, and a growth factor may be deposited on the carrier. Comminuting is performed by digesting with an enzyme and/by mechanically comminuting. Liquid used to form the suspension may contain a gel precursor which is gelled after the suspension is deposited to the carrier. Preferably, the implanting material is implanted within about 1 hour of removing the fatty tissue from the patient.

Claims

1. A process for the forming and applying an implant material with bone regenerating characteristics to form bone in a patient, comprising:

removing fatty tissue containing no osteoblasts or mesanchymal cells derived from bone, bone marrow or periosteum from a patient in need of bone regeneration;

comminuting said fatty tissue material to produce small fatty tissue particles;

suspending at least some of the small fatty tissue particles in a physiologically acceptable, liquid to form a suspension comprising the particles;

depositing at least a substantial portion of the suspended fatty tissue particles from said suspension on a physiologically acceptable, solid carrier material by contacting the suspension with the carrier material to form a solid implanting material;

implanting said solid implanting material into a bone in need of regeneration in a patient; and

allowing bone to regenerate in the patient,

wherein said removing of said fatty tissue and implanting of said implanting material are carried out in at most about 1 hour of elapsed time.

2. The process as claimed in claim 1 wherein said fatty tissue is not cultured between removal thereof from said patient and implantation thereof into said patient.

3. The process as claimed in claim 1 further wherein said solid carrier material is pulverulent.

4. The process as claimed in claim 1 wherein said solid carrier material has a form selected from the group consisting of sponge form, textile form, porous particle form, and monolith form.

5. The process as claimed in claim 1 further comprising filtering said suspension through said solid carrier material to deposit at least a substantial portion of said particles on said carrier material.

6. The process as claimed in claim 1 further comprising applying a cell adhesion agent to said solid carrier material prior to completion of contact thereof with said suspension.

7. The process as claimed in claim 1 further comprising admixing a cell adhesion agent with said suspension prior to contacting said suspension with said carrier material.

8. The process as claimed in claim 1 wherein said suspending liquid comprises a gel precursor, and said gel precursor is gelled after said tissue particles are deposited on said carrier material.

9. The process as claimed in claim 1 further comprising mechanically comminuting said fat tissue.

10. The process as claimed in claim 1 further comprising comminuting said tissue by digesting said fatty tissue with at least one enzyme.

11. The process as claimed in claim 9 further comprising, in addition to said mechanical comminution, comminuting said fatty tissue by digesting said fatty tissue with at least one enzyme.

12. The process as claimed in claim 1 wherein said solid carrier material comprises at least one material selected from the group consisting of demineralized bone, collagen, mineral material, and synthetic polymer material.

13. The process as claimed in claim 11 further comprising depositing at least one growth factor on said carrier material with said fatty tissue particles deposited thereon.

14. A process for forming and applying an implant material with cartilage regenerating characteristics to form cartilage in a patient, comprising:

removing fatty tissue material containing no chondrocytes or other cartilage forming cells derived from cartilage, bone marrow or periosteum from a patient in need of cartilage regeneration;

comminuting said fatty tissue to produce small fatty tissue particles;

suspending at least some of said small fatty tissue particles in a physiologically acceptable, liquid to form a suspension comprising the fatty tissue particles;

depositing at least a substantial portion of said fatty tissue particles from said suspension on a physiologically acceptable, solid carrier, by contacting the suspension with the carrier material to form a solid implanting material;

implanting said solid implanting material into a cartilage in need of regeneration in a patient; and

allowing cartilage to regenerate in the patient;

wherein said removing of the fatty tissue and implanting of said implanting material are carried out collectively in at most about 1 hour.

15. The process as claimed in claim 14 wherein said fatty tissue is not cultured between removal thereof from said patient and implantation thereof into said patient.

16. The process as claimed in claim 14 wherein said solid carrier material is pulverulent.

17. The process as claimed in claim 14 wherein said solid carrier material has a form selected from the group consisting of sponge form, textile form, porous particles, and monolith form.

18. The process as claimed in claim 14 comprising filtering said suspension through said solid carrier material to deposit at least a substantial portion of said fatty tissue particles on the carrier material.

19. The process as claimed in claim 14 further comprising applying a cell adhesion agent to said solid carrier material prior to completion of contact thereof with said suspension.

20. The process as claimed in claim 14 further comprising admixing a cell adhesion agent with said suspension prior to contacting said suspension with said carrier material.

21. The process as claimed in claim 14 wherein said suspending liquid comprises a gel precursor, and said gel precursor is gelled after the fatty tissue particles are deposited on the carrier material.

22. The process as claimed in claim 14 further comprising mechanically comminuting said fatty tissue.

23. The process as claimed in claim 14 further comprising comminuting said tissue by digesting said fatty tissue with at least one enzyme.

24. The process as claimed in claim 22 further comprising, in addition to said mechanical comminution, comminuting said tissue by digesting said fatty tissue with at least one enzyme.

25. The process as claimed in claim 14 wherein said solid carrier material comprises at least one material selected from the group consisting of demineralized bone, collagen, mineral material, and synthetic polymer.

26. The process as claimed in claim 14 further comprising depositing at least one growth factor on said carrier material with said fatty tissue particles deposited thereon.