Devices and methods for detecting nucleic acid analytes in samples

• US 5,837,466 A
• Filed: 12/16/1996
• Issued: 11/17/1998
• Est. Priority Date: 12/16/1996
• Status: Expired due to Term

• Current Assignee: Abbott Molecular Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Vysis, Inc. (Abbott Laboratories Incorporated)
• Inventors: Lane, David J., Farrell, Michael P.
• Primary Examiner(s): LeGuyader, John L.
• Assistant Examiner(s): LARSON, THOMAS GEORGE
• Application Number:
  US08/768,177
• Time in Patent Office: 701 Days
• Field of Search: 435/6, 435/91.2, 435/91.21, 435/91.31, 435/91.3, 435/91.52, 436/94, 536/24.3, 935/76, 935/77
• US Class Current: 435/6.19
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2531/149 Replicase based amplification, e.g. Q beta replicase
• CPC Class Codes: C12Q 2561/125 Ligase Detection Reaction [LDR]
• CPC Class Codes: C12Q 2565/537 characterised by the capture oligonucleotide acting as a primer

Abstract

The invention provides devices and methods for use in detecting nucleic acid analytes in samples. The devices each include a solid support to which is bound a two-dimensional distribution or field of nucleic acid probes that each bind to a nucleic acid analyte, which is detected by use of amplification methods.

Claims

1. An assay device for detecting the presence of a nucleic acid analyte in a sample, said device comprising a planar, solid support having covalently bound thereto a two-dimensional field of analyte-specific nucleic acid probes, each probe comprising a first end covalently bound to said support and including a 5'"'"' portion of an autocatalytically replicable nucleic acid, and a second end including an analyte-binding segment.

2. The device of claim 1, wherein said autocatalytically replicable nucleic acid is replicable by Q-beta replicase.

3. The device of claim 2, wherein said autocatalytically replicable nucleic acid comprises a midivariant nucleic acid.

4. The device of claim 1, wherein said solid support is a glass plate.

5. A method for detecting the presence of a nucleic acid analyte in a sample, said method comprising the steps of:

(a) applying the sample to a solid support having covalently bound thereto a two-dimensional field of first analyte-specific nucleic acid probes, each of the first probes comprising a first end bound to the solid support and including a 5'"'"' portion of an autocatalytically replicable nucleic acid, and a second end including a first analyte-binding segment, wherein the first analyte-binding segment of each of the first probes hybridizes to a first region of the analyte;

(b) applying a second nucleic acid probe to the solid support, the second probe comprising a second analyte-binding segment that hybridizes to a second region of the analyte, the second probe further comprising a remainder of said autocatalytically replicable nucleic acid, wherein the first and second regions of the analyte are adjacent nucleotide segments, and wherein the analyte, the first probe, and the second probe hybridize together on the support to form a complex including a complete autocatalytically replicable nucleic acid;

(c) applying a diffusion limiting matrix to the solid support;

(d) amplifying the complete autocatalytically replicable nucleic acid to generate an amplified product; and

(e) detecting the amplified product as a measure of the presence of the analyte in the sample.

6. The method of claim 5, further comprising the step of ligating the first and second probes in said complex together to form said complete autocatalytically replicable nucleic acid.

7. The method of claim 5, wherein the sample is applied to the solid support prior to being contacted with the second probe.

8. The method of claim 5, wherein the sample is contacted with the second probe prior to being applied to the solid support.

9. The method of claim 5, wherein the amplification step is carried out in the presence of a fluorescent intercalating dye and the detection step is carried out by monitoring the amplified product for the presence of the dye.

10. The method of claim 5, wherein the detection step is carried out by employing a fluor-quencher pair.

11. The method of claim 5, wherein multiple analytes are simultaneously detected in the sample and the detection step is carried out by employing multiple fluor-quencher pairs that comprise distinguishable fluorescent moieties.

12. The method of claim 5, wherein said autocatalytically replicable nucleic acid comprises a nucleic acid replicable by Q-beta replicase.

13. The method of claim 12, wherein said autocatalytically replicable nucleic acid comprises a midivariant nucleic acid.

14. The method of claim 5, further comprising washing said complex to remove any unbound first or second probes.

15. The method of claim 5, wherein said diffusion limiting matrix is selected from the group consisting of gelatin, agarose, polyacrylamide, and any combination thereof.

16. The method of claim 5, further comprising determining the concentration of the analyte in the sample.

17. The method of claim 5, wherein the amplification step generates a colony of amplified product, the colony being centered around the complete autocatalytically replicable nucleic acid;

and wherein the concentration of the analyte in the sample is determined by counting colonies of amplified product formed on the solid support, and the number of colonies corresponds to the concentration of the analyte in the sample.

18. An assay system for detecting a nucleic acid analyte in a sample, said assay system comprising:

(a) a planar solid support having covalently bound thereto a two-dimensional field of first analyte-specific nucleic acid probes, each of the first probes comprising a first end covalently bound to the solid support and including a 5'"'"' portion of an autocatalytically replicable nucleic acid, and a second end including a first analyte-binding segment, wherein the first analyte-binding segment of each of the first probes hybridizes to a first region of the analyte; and

(b) a second nucleic acid probe comprising a second analyte-binding segment that hybridizes to a second region of the analyte, the second probe further comprising a remainder of the autocatalytically replicable nucleic acid, and the first and second regions of the analyte comprising adjacent nucleotide segments;

wherein binding of the first and second probes to the analyte permits amplification of the autocatalytically replicable nucleic acid.

19. An assay system for detecting a nucleic acid analyte in a sample, said assay system comprising:

(a) a solid support having bound thereto a two-dimensional field of capture probes, each of the capture probes comprising a capture segment that hybridizes to a capture region of the analyte;

(b) a first nucleic acid probe comprising a first segment that hybridizes to a first region of the analyte;

(c) a second nucleic acid probe comprising a second segment that hybridizes to a second region of the analyte; and

(d) a diffusion limiting matrix for application to the solid support;

wherein binding of the capture probes and the first and second probes to the analyte permits amplification of a detectable product within the diffusion limiting matrix.

20. The assay system of claim 19, wherein the first and second regions of the analyte are adjacent nucleotide segments, said first nucleic acid probe further comprises a portion of an autocatalytically replicable nucleic acid molecule, and said second nucleic acid probe further comprises a remaining portion of said autocatalytically replicable nucleic acid molecule.

21. The assay system of claim 19, wherein the capture and first regions of the analyte are adjacent nucleotide segments and the capture and second regions of the analyte are adjacent nucleotide segments, said first nucleic acid probe further comprises a portion of an autocatalytically replicable nucleic acid molecule, and said second nucleic acid probe further comprises a remaining portion of said autocatalytically replicable nucleic acid molecule.

22. The method for detecting the presence of a nucleic acid analyte in a sample, said method comprising the steps of:

(a) contacting the sample with the bound capture probes and the first and second probes of the assay system of claim 19, wherein the first and second regions of the analyte are adjacent nucleotide segments, the first probe further comprises a portion of an autocatalytically replicable nucleic acid molecule, the second probe further comprises a remaining portion of the autocatalytically replicable nucleic acid molecule, and the contacting permits the analyte, the first probe, and the second probe to hybridize together on the solid support to form a complex including a complete autocatalytically replicable nucleic acid;

(b) applying a diffusion limiting matrix to the solid support;

(c) amplifying the complete autocatalytically replicable nucleic acid to generate an amplified product; and

(d) detecting the amplified product as a measure of the presence of the analyte in the sample.

23. A method for detecting the presence of a nucleic acid analyte in a sample, said method comprising the steps of:

(a) contacting the sample with the bound capture probes and the first and second probes of the assay system of claim 19, wherein the capture and first regions of the analyte are adjacent nucleotide segments and the capture and second regions of the analyte are adjacent nucleotide segments, the first probe further comprises a portion of an autocatalytically replicable nucleic acid molecule, and the second probe further comprises a remaining portion of the autocatalytically replicable nucleic acid molecule, and the contacting permits the analyte, the first probe, and the second probe to hybridize together on the said support to form a complex including a complete autocatalytically replicable nucleic acid;

(b) applying a diffusion limiting matrix to the solid support;

(c) amplifying the complete autocatalytically replicable nucleic acid to generate an amplified product; and

(d) detecting the amplified product as a measure of the presence of the analyte in the sample.

24. A method for detecting the presence of a nucleic acid analyte in a sample, the method comprising the steps of:

(a) contacting the sample with a solid support having covalently bound thereto a two-dimensional field of first analyte-specific nucleic acid probes, each of the first probes comprising a first end bound to the support and including a second end including a first analyte-binding segment;

(b) applying a diffusion limiting matrix to the support;

(c) performing amplification by one of polymerase chain reaction, ligase chain reaction, transcription-mediated amplification, nucleic acid sequence-based amplification, and strand displacement amplification in a manner dependent upon the presence of both the first probe and the nucleic acid analyte, and in such a manner that the products of such amplification reaction are restrained to form localized foci within the diffusion limiting matrix on said support; and

(d) detecting the amplified products as a measure of the presence of the analyte in the sample.

25. An assay system for detecting a nucleic acid analyte in a sample, said assay system comprising:

(a) a solid support having covalently bound thereto a two-dimensional field of first analyte-specific nucleic acid probes, each of the first probes comprising a first end covalently bound to the solid support and including a 5'"'"' portion of an autocatalytically replicable nucleic acid, and a second end including a first analyte-binding segment, wherein the first analyte-binding segment of each of the first probes hybridizes to a first region of the analyte;

(b) a second nucleic acid probe comprising a second analyte-binding segment that hybridizes to a second region of the analyte, the second probe further comprising a remainder of the autocatalytically replicable nucleic acid, and the first and second regions of the analyte comprising adjacent nucleotide segments; and

(c) a diffusion limiting matrix for application to the solid support;

wherein binding of the first and second probes to the analyte permits amplification of the autocatalytically replicable nucleic acid within the diffusion limiting matrix.