Patched genes and their use

• US 5,837,538 A
• Filed: 10/06/1995
• Issued: 11/17/1998
• Est. Priority Date: 10/07/1994
• Status: Expired due to Term

• Current Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford University)
• Original Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford University)
• Inventors: Scott, Matthew P, Johnson, Ronald L., Goodrich, Lisa V.
• Primary Examiner(s): Chambers, Jasemine C.
• Assistant Examiner(s): MARTIN, JILL D
• Application Number:
  US08/540,406
• Time in Patent Office: 1,138 Days
• Field of Search: 536/23.1, 536/23.5, 536/24.31, 435/172.3, 435/69.1, 435/325, 435/91.2, 435/320.1, 424/93.21
• US Class Current: 435/325
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/43563 from insects
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

Invertebrate and vertebrate patched genes are provided, including the mouse and human patched genes, as well as methods for isolation of related genes, where the genes may be of different species or in the same family. Having the ability to regulate the expression of the patched gene, allows for the elucidation of embryonic development, cellular regulation associated with signal transduction by the patched gene, the identification of agonist and antagonist to signal transduction, identification of ligands for binding to patched, isolation of the ligands, and assaying for levels of transcription and expression of the patched gene.

Claims

1. An isolated nucleic acid comprising a ptc coding sequence for a naturally occurring vertebrate patched polypeptide, or allelic variant thereof, wherein the ptc coding sequence hybridizes which binds to a hedgehog polypeptide to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 5×

SSC at 60°

C.

2. The nucleic acid of claim 1, wherein the ptc coding sequence hybridizes to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 0.1×

SSC at 50°

C.

3. The nucleic acid of claim 1, or 2, wherein the ptc coding sequence encodes a mammalian patched polypeptide.

4. The nucleic acid of claim 3, wherein the coding sequence encodes a primate patched polypeptide.

5. The nucleic acid of claim 4, wherein the coding sequence encodes a human patched polypeptide.

6. The nucleic acid of claim 1, wherein the amino acid sequence of the ptc polypeptide is identical to SEQ ID No. 10 or 19.

7. The nucleic acid of claim 1, wherein the ptc coding sequence is identical to SEQ ID No. 9 or 18.

18. The nucleic acid of claim 1, or 8, further comprising a heterologous transcription initiation region that controls transcription of the coding sequence.

19. The nucleic acid of claim 18, wherein the transcription initiation region is inducible.

20. The nucleic acid of claim 18, wherein the transcription initiation region includes a promoter.

29. A cell comprising and expressing the nucleic acid of claim 1, 8, 17, or 21.

30. The cell of claim 29, which cell is a eukaryotic cell.

31. The cell of claim 30, which cell is mammalian cell.

8. An isolated nucleic acid comprising a coding sequence for a polypeptide including a hedgehog-binding sequence that binds hedgehog protein and which is encoded by a nucleotide sequence that hybridizes to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 5×

SSC at 60°

C, wherein the polypeptide retains hedgehog-binding activity.

9. The nucleic acid of claim 8, wherein the hedgehog-binding sequence is encoded by a nucleotide sequence that hybridizes to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 0.1 ×

SSC at 60°

C.

10. The nucleic acid of claim 8, or 9, wherein the hedgehog-binding sequence is from a mammalian patched polypeptide.

11. The nucleic acid of claim 10, wherein the hedgehog-binding sequence is from a primate patched polypeptide.

12. The nucleic acid of claim 11, wherein the hedgehog-binding sequence is from a human patched polypeptide.

13. The nucleic acid of claim 8, wherein the nucleotide sequence encoding the hedgehog-binding sequence is at least 267 base pairs in length.

14. The nucleic acid of claim 8, wherein the nucleotide sequence encoding the hedgehog-binding sequence is at least 345 base pairs in length.

15. The nucleic acid of claim 14, wherein the hedgehog-binding sequence includes at least 3 extracellular loops.

16. The nucleic acid of claim 8, wherein the hedgehog protein is selected from the group consisting of Sonic hedgehog, Indian hedgehog, and Desert hedgehog.

32. The nucleic acid of claim 13, wherein the nucleotide sequence encoding the hedgehog-binding sequence is at least 1 Kbp in length.

17. A nucleic acid comprising:

(i) a coding sequence from a naturally occurring vertebrate ptc gene or allelic variant thereof, which coding sequence (a) hybridizes to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 5×

SSC at 60°

C. and (b) encodes a polypeptide that binds hedgehog proteins, and (ii) a heterologous transcriptional initiation region controlling the transcription of the coding sequence.

22. The nucleic acid of claim 17, wherein the coding sequence hybridizes to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 0.1×

SSC at 50°

C.

23. The nucleic acid of claim 17, wherein the amino acid sequence of the ptc polypeptide is identical to SEQ ID No. 10 or19.

24. The nucleic acid of claim 17, wherein the coding sequence is identical to SEQ ID No. 9 or 18.

21. A nucleic acid comprising:

(i) a coding sequence for a polypeptide including a hedgehog-binding sequence that (a) binds hedgehog protein and (b) is encoded by a nucleotide sequence that hybridizes to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 5×

SSC at 60°

C., and (ii) a heterologous transcriptional initiation region controlling the transcription of the coding sequence, wherein the polypeptide retains hedgehog-binding activity.

25. The nucleic acid of claim 21, wherein the hedgehog-binding sequence is encoded by a nucleotide sequence that hybridizes to the complement of the coding sequence of SEQ ID Nos. 9 or 18 under stringency conditions equivalent to 0.1×

SSC at 60°

C.

26. The nucleic acid of claim 21, wherein the coding sequence encodes a mammalian patched polypeptide.

27. The nucleic acid of claim 26, wherein the coding sequence encodes a primate patched polypeptide.

28. The nucleic acid of claim 27, wherein the coding sequence encodes a human patched polypeptide.