Antibody construct
1 Assignment
0 Petitions
Accused Products
Abstract
An antigen binding protein construct or "minibody" which includes the VL and VH domains of a native antibody fused to the hinge region and CH3 domain of the immunoglobulin molecule is described. Minibodies are small versions of whole antibodies which encode in a single chain the essential elements of a whole antibody. Minibodies are expressed by host cells transformed with minibody genes.
217 Citations
19 Claims
- 1. A minibody dimer as depicted by FIG. 2.
-
2. A minibody consisting essentially of the light and heavy chain variable domains of a native antibody fused to hinge and CH3 domains of an immunoglobulin molecule as depicted by FIG. 1.
-
3. The minibody consisting essentially of the light and heavy chain variable domains of the T84.66 CEA antibody fused to the hinge and CH3 domains of human IgG.
- 4. The T84.66 minibody depicted by FIG. 3C.
-
6. A method for constructing a minibody as depicted by FIG. 1, which comprises:
-
(i) providing a single chain antibody including the variable light chain and variable heavy chain domains of a native antibody, and (ii) fusing said single chain antibody to a construct of a native antibody hinge region and CH3 domain. - View Dependent Claims (7, 8, 9)
-
-
10. The single chain T84.66 CEA antibody depicted by FIG. 3A (SEQ ID NO:
- 1).
- 11. A method of producing a minibody depicted by FIG. 1 or FIG. 2 which comprises transforming a host cell with an expression vector containing the nucleic acid sequence of a minibody gene and culturing the transformed host cell under conditions which permit expression of said nucleic acid sequence by said host cell.
- 12. A method of producing a T84.66 minibody as depicted by FIG. 3C which comprises transforming a host cell with an expression vector containing the nucleic acid sequence of said minibody and culturing the transformed host cell under conditions which permit expression of said nucleic acid sequence by the host cell.
- 15. A minibody consisting essentially of the light and heavy chain variable domains of an antibody fused to, in sequence, the hinge region of an antibody, an amino acid linker and the CH3 domain of an immunoglobulin molecule.
-
17. A minibody consisting essentially of
(a) a first single chain construct consisting essentially of a light chain variable region joined to a heavy chain variable region which is fused, in sequence, to hinge and CH3 domains of an immunoglobulin molecule, and (b) a second single chain construct consisting essentially of a light chain variable region joined to a heavy chain variable region which is fused to, in sequence, a hinge and CH3 domains of an immunoglobulin molecule, wherein said first single chain construct is linked to said second single chain construct by disulfide bonds between the hinge regions.
-
18. A minibody consisting essentially of
(a) a first single chain construct consisting essentially of a light chain variable region joined to a heavy chain variable region which is fused, in sequence, to a hinge region, an amino acid linker and a CH3 domain of an immunoglobulin molecule, and (b) a second single chain construct consisting essentially of a light chain variable region joined to a heavy chain variable region which is fused, in sequence, to a hinge region, an amino acid linker and a CH3 domain of an immunoglobulin molecule, wherein said first single chain construct is linked to said second single chain construct by disulfide bonds between the hinge regions.
Specification