High affinity HKGF nucleic acid ligands and inhibitors
First Claim
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1. A nucleic acid ligand to Human Keratinocyte Growth Factor (hKGF) identified according to the method comprising:
- a) preparing a candidate mixture of nucleic acids;
b) contacting the candidate mixture of nucleic acids with hKGF, wherein nucleic acids having an increased affinity to hKGF relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to hKGF, whereby a nucleic acid ligand of hKGF may be identified.
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Abstract
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to hKGF. Included in the invention are specific RNA ligands to hKGF identified by the SELEX method. Also included are RNA ligands that inhibit the interaction of hKGF with its receptor.
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13 Claims
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1. A nucleic acid ligand to Human Keratinocyte Growth Factor (hKGF) identified according to the method comprising:
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a) preparing a candidate mixture of nucleic acids; b) contacting the candidate mixture of nucleic acids with hKGF, wherein nucleic acids having an increased affinity to hKGF relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to hKGF, whereby a nucleic acid ligand of hKGF may be identified. - View Dependent Claims (4, 5, 6, 7, 8)
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2. A purified and isolated non-naturally occurring RNA ligand to hKGF wherein said ligand is selected from the group consisting of the sequences set forth in Table 3 (SEQ ID NOS. 4-77).
- 3. A purified and isolated non-naturally occurring nucleic acid ligand to hKGF.
Specification