Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein

• US 5,837,847 A
• Filed: 08/29/1997
• Issued: 11/17/1998
• Est. Priority Date: 06/30/1994
• Status: Expired due to Term

• Current Assignee: Novozymes
• Original Assignee: NOVO Nordisk Biotech Incorporated (Novo Nordisk A/S)
• Inventors: Royer, John C., Wendy, Yoder T., Moyer, Donna L., Shuster, Jeffrey R.
• Primary Examiner(s): Railey, Johnny F.
• Application Number:
  US08/921,426
• Time in Patent Office: 445 Days
• Field of Search: 536/24.1, 435/929
• US Class Current: 536/24.1
• CPC Class Codes: C12N 15/80 for fungi
• CPC Class Codes: C12N 9/20 Triglyceride splitting, e.g. by means of lipase
• CPC Class Codes: C12N 9/58 derived from fungi
• CPC Class Codes: C12N 9/6427 Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
• CPC Class Codes: C12P 21/00 Preparation of peptides or proteins single cell protein C12N1/00
• CPC Class Codes: C12P 21/02 having a known sequence of two or more amino acids, e.g. glutathione
• CPC Class Codes: Y10S 435/929 Fusarium

Abstract

The invention is related to a non-toxic, non-toxigenic, non-pathogenic recombinant Fusarium, e.g., Fusarium graminearum host cell comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter. The invention further relates to a method for the production of recombinant proteins using such Fusarium host cells. The invention also relates to a promoter and terminator sequence which may be used in such cells.

Claims

1. A fungal promoter which is derived from a gene encoding a Fusarium oxysporum trypsin-like protease or a fragment thereof having the same promoter activity as the promoter sequence shown in SEQ ID NO:

5, in which the general sequence encoding said protease is shown in SEQ ID NO;

3.

2. The fungal promoter of claim 1 in which said promoter has a DNA sequence depicted in SEQ ID NO:

5.

3. A fungal terminator which is derived from a gene encoding a Fusarium oxysporum trypsin-like protease or a fragment thereof having the same terminator activity as the terminator sequence shown in SEQ ID NO:

6, in which the general sequence encoding said protease is shown in SEQ ID NO;

3.

4. The fungal terminator of claim 3 in which said terminator has a DNA sequence depicted in SEQ ID NO:

6.