Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
First Claim
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1. A method for the covalent attachment of nucleic acid molecules to a solid-phase, which comprises the steps of:
- A) coating a solid-phase surface with a mercaptosilane wherein said mercaptosilane comprises sulfhydryl groups; and
B) coupling a sulfhydryl or disulfide modified nucleic acid molecule to the sulfhydryl groups of said mercaptosilane by means of a covalent disulfide bond.
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Abstract
Methods for the covalent, specific and reversible immobilization of nucleic acid molecules onto solid-phases by means of a reversible disulfide bond for nucleic acid molecule array preparation are described. These methods can be used to prepare reusable nucleic acid molecule arrays with high specificity and high efficiency.
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Citations
27 Claims
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1. A method for the covalent attachment of nucleic acid molecules to a solid-phase, which comprises the steps of:
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A) coating a solid-phase surface with a mercaptosilane wherein said mercaptosilane comprises sulfhydryl groups; and B) coupling a sulfhydryl or disulfide modified nucleic acid molecule to the sulfhydryl groups of said mercaptosilane by means of a covalent disulfide bond. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification