Plate for reaction system

• US 5,840,256 A
• Filed: 04/09/1996
• Issued: 11/24/1998
• Est. Priority Date: 04/09/1996
• Status: Expired due to Fees

• Current Assignee: Sarnoff Corporation (SRI International, Inc.)
• Original Assignee: David Sarnoff Research Center, Inc.
• Inventors: Levine, Aaron Wiliam, O'Mara, Kerry Dennis, Cherukuri, Satyam Choudary, Demers, Robert Richard
• Primary Examiner(s): Pyon, Harold Y.
• Application Number:
  US08/630,018
• Time in Patent Office: 959 Days
• Field of Search: 422/100-104, 422/82.05, 356/246, 356/427, 356/440, 204/299 R, 204/600, 204/601, 436/809
• US Class Current: 422/552
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

The invention provides a plate having a plurality uniformly sized reaction cells formed in its upper surface, wherein the density of the reaction cells is at least about 10 cells per cm². Preferably, the area of each of the openings of the reaction cells is no more than about 55% of the area defined by the multiplication product of (1) the pitch between reaction cells in separate rows and (2) the pitch between reaction cells in separate columns.

Claims

1. A processing system for conducting a plurality of processes in parallel, the processing system comprising

a liquid distribution system for directing liquids to a plurality of uniformly sized reaction cells, and

a plate comprising said cells, the plate is reversibly and sealably attached to the liquid distribution system, having a plurality of the reaction cells formed in its upper surface and having seal-forming surfaces surrounding the reaction cells for sealing with the liquid distribution system and thereby isolating reaction cells from adjacent reaction cells, wherein the density of the reaction cells is at least about 10 cells per cm², wherein reaction cells are separated from each adjacent reaction cell by at least about 0.15 mm,

wherein the plate can engage the liquid distribution system such that a plurality of said cells can receive liquid from the liquid distribution system without having to adjust the engagement between the plate and the liquid distribution system and wherein the area of each of the openings of the reaction cells of the plate is no more than about 50% of the area defined by the multiplication product of (1) the pitch between reaction cells in separate rows and (2) the pitch between reaction cells in separate columns.

2. The processing system of claim 1, wherein the cells are arrayed in rows and columns.

3. The processing system of claim 1, wherein the plate is rectangular.

4. The processing system of claim 1, wherein the area of each of the openings of the reaction cells is no more than about 45% of the area defined by the multiplication product of (1) the pitch between reaction cells in separate rows and (2) the pitch between reaction cells in separate columns.

5. The processing system of claim 1, wherein the reaction cells are rectangular with a width of about 400 microns to about 1200 microns.

6. The processing system of claim 1, wherein the reaction cells have a depth of about 200 microns to about 400 microns.

7. The processing system of claim 1, wherein the density of cells is at least about 20 cells per cm².

8. The processing system of claim 1, wherein the density of cells is at least about 40 cells per cm².

9. The processing system of claim 1, wherein the pitch between reaction cells in a row or column is at least about 0.5 mm.

10. The processing system of claim 1, wherein each reaction cell is separated from each adjacent reaction cell by at least about 0.3 mm.

11. The processing system of claim 1, wherein each reaction cell has a substantially square shape.

12. The processing system of claim 1, wherein the plate has at least about 1,000 reaction cells.

13. The processing system of claim 1, wherein the plate has at least about 4,000 reaction cells.

14. The processing system of claim 1, further comprising two optical reference structures for orienting the reaction cells with respect to the liquid distribution system or a detection device designed to operate with the plate.

15. The processing system of claim 1, wherein liquid distribution system operates to addressably direct a plurality of liquids to the plurality of reaction cells.

16. A method of conducting a plurality of reactions in parallel in the processing system of claim 15, comprising

operating the liquid distribution system for addressably directing a plurality of liquids to the plurality of cells, wherein the plate with the wells is reversibly sealed with a defined alignment to the liquid distribution system.

17. The processing system of claim 1, further comprising a first alignment marker on a first edge of the plate and a second alignment marker on a second edge of the plate perpendicular to the first edge, wherein the first and second alignment markers are for orienting the reaction cells with a liquid distribution system or a detection device designed to operate with the plate.

18. The processing system of claim 12, wherein the first and second alignment markers are notches at the edges of the plate designed to interact with locating pins used to mechanically orient the reaction cells.

19. The processing system of claim 12, further comprising a third alignment marker on the second edge, wherein the third alignment marker is for orienting the reaction cells with the liquid distribution system or a detection device designed to operate with the plate and is offset from the second alignment marker.

20. The processing system of claim 19, wherein the first, second and third alignment markers are notches designed to interact with locating pins used to mechanically orient the reaction cells.

21. The processing system of claim 20, wherein the optical reference structures are etched into the plate.