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Comparative gene transcript analysis

  • US 5,840,484 A
  • Filed: 01/27/1994
  • Issued: 11/24/1998
  • Est. Priority Date: 07/17/1992
  • Status: Expired due to Fees
First Claim
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1. A method of analyzing a specimen containing gene transcripts, comprising the steps of:

  • obtaining a first mixture of mRNA;

    making cDNA copies of the mRNA;

    isolating a representative population of the cDNA copies and producing therefrom a first cDNA library, wherein a selected set of random primers is used in the generation of the first cDNA library;

    identifying a first set of gene transcripts from the first library and determining cDNA sequences corresponding to the gene transcripts;

    processing the cDNA sequences corresponding to the first gene transcripts in a programmed computer in which a database of reference transcript sequences indicative of reference cDNA sequences is stored, to generate a first identified sequence value for each of the first gene transcripts, where each said identified sequence value is indicative of a sequence annotation and a degree of match between one of the first gene transcripts and at least one of the reference cDNA sequences; and

    processing each said identified sequence value to generate first final data values indicative of a number of times each first identified sequence value is present in the first cDNA library.

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