Method for separating sex specific molecules and non-sex specific molecules
First Claim
1. A method for separating sex specific molecules and non-sex specific molecules associated with animal plasma membranes, comprising:
- (a) treating a plasma membrane fraction from adult, fetal, or embryonic animal cells or tissues, with one or more antibodies which bind to non-sex specific molecules in the cell membrane fraction to form conjugates between the non-sex specific molecules and the antibodies; and
(b) separating the material in the cell membrane fraction which does not bind to the antibodies to obtain a subfraction containing sex specific molecules.
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Abstract
A method for identifying non-sex specific and sex specific molecules associated with animal cell membranes is described. The method involves preparing a cell membrane fraction from adult, fetal, or embryonic animal cells; treating the cell membrane fraction with one or more substances which bind to non-sex specific molecules in the cell membrane fraction to form conjugates between the non-sex specific molecules and the substances; separating the material in the cell membrane fraction which does not bind to the substances to obtain a subfraction containing sex specific molecules; optionally, removing the substances in the conjugates to obtain a subfraction containing non-sex specific molecules; and, isolating the sex specific molecules and optionally non-sex specific molecules in the subfractions. Sex specific and non-sex specific molecules and methods of using the sex specific and non-sex specific molecules for sexing are also described.
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Citations
7 Claims
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1. A method for separating sex specific molecules and non-sex specific molecules associated with animal plasma membranes, comprising:
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(a) treating a plasma membrane fraction from adult, fetal, or embryonic animal cells or tissues, with one or more antibodies which bind to non-sex specific molecules in the cell membrane fraction to form conjugates between the non-sex specific molecules and the antibodies; and (b) separating the material in the cell membrane fraction which does not bind to the antibodies to obtain a subfraction containing sex specific molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification