Phenotypic characterization of the hematopoietic stem cell
First Claim
1. A population of human cells that lacks mature progenitor cells of the erythroid, lymphoid, and myeloid lineages and is enriched for hematopoietic stem cells wherein said stem cells are capable of limited self-renewal and differentiation into erythroid myeloid and lymphoid progenitors and mature cells, which population is comprised of cells having a CD34+ /CD38- /HLA-DR+ phenotype, wherein cells having said phenotype comprise at least 95% of said population.
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Abstract
One or more population of cells enriched for human hematopoietic stem cells is disclosed. HSC in this population of cells are capable of limited self-renewal and are capable of differentiating into all elements of the hematopoietic system. This population of cells has the phenotype of CD34+ /CD38- and more preferably CD34+ /CD38- /HLA-DR+. Cells within this population have been found to express CD13, CD33 and CD71. Hematopoietic stem cells can be used in a number of therapies, including autologous transplantation and in gene therapy.
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Citations
9 Claims
- 1. A population of human cells that lacks mature progenitor cells of the erythroid, lymphoid, and myeloid lineages and is enriched for hematopoietic stem cells wherein said stem cells are capable of limited self-renewal and differentiation into erythroid myeloid and lymphoid progenitors and mature cells, which population is comprised of cells having a CD34+ /CD38- /HLA-DR+ phenotype, wherein cells having said phenotype comprise at least 95% of said population.
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5. A population of human cells comprising hematopoietic stem cells wherein said human cells are of a phenotype CD34+ /CD38- /HLA-DR+ /CD13+ /CD33+ /CD71+, wherein cells of the phenotype comprise at least 95% of said population.
- 6. A method of isolating a population of human cells that lacks mature and progenitor cells of the erythroid, lymphoid and myeloid lineages and enriched for hematopoietic stem cells comprising the steps of obtaining human tissue containing hematopoietic stem cells, selecting said hematopoietic cells based upon the expression of at least CD34, HLA-DR, and CD38 antigens, and separating the cells that express CD34 and HLA-DR but do not express CD38, wherein said CD34+ /HLA-DR+ /CD38- cells comprise at least 95% of said population.
Specification