Method for performing spectroscopic analysis of inhomogeneous test samples

• US 5,841,523 A
• Filed: 04/20/1995
• Issued: 11/24/1998
• Est. Priority Date: 04/20/1995
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Chiron Corporation (Bayer AG)
• Inventors: Garyantes, Michael F., Degen, Beat R.
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): VIERRA EISENBERG, JASON D
• Application Number:
  US08/425,559
• Time in Patent Office: 1,314 Days
• Field of Search: 356/300-334, 356/73, 356/39-40, 356/72, 364/571.01, 364/571.04, 364/498, 364/570, 364/574, 364/578
• US Class Current: 356/72
• CPC Class Codes: G01N 21/27 using photo-electric detection G01N21/31 takes precedence; circuits for computing concentration logarithmic circuits G06G7/24; photometric circuits in general G01J

Abstract

A method is provided for more accurately calculating the concentration of analytes or components in test samples. The method includes the use of an algorithm for analysis, and selection of sets of signal measurements from a spectrophotometer. Sets of signal measurements are selected to avoid inhomogeneities, i.e. air bubbles, artifacts and dilution, in test samples being analyzed. The method has application to the spectroscopic determination of the concentration of hemoglobin derivatives or fractions in a blood sample.

Claims

1. A method for spectroscopic analysis of fluid test samples comprising:

(a) providing a test sample for spectroscopic analysis to an optical cell;

(b) performing a plurality of signal measurements on said test sample;

(c) selecting from said signal measurements one or more sets of measurements having both low variability relative to the range of variation of the signal measurements or a defined threshold, and either signal level maxima or signal level minima; and

(d) analyzing the test sample utilizing said one or more selected set of signal measurements.

2. The method, as provided in claim 1, wherein said signal measurements are selected by performing a temporal scan or a spatial scan of the test sample.

3. The method, as provided in claim 1, wherein a single set of signal measurements or window is selected to analyze the test sample.

4. The method, as provided in claim 1, wherein said signal measurements include either transmittance, absorbance, or reflectance measurements.

5. The method, as provided in claim 3, wherein the mean value of said set of signal measurements is utilized to analyze the test sample.

6. The method, as provided in claim 1, wherein said signal measurements are performed serially over consecutive time intervals.

7. The method, as provided in claim 1, wherein said plurality of signal measurements are performed in a period of time less than or equal to ten seconds.

8. The method, as provided in claim 6, wherein each signal measurement is performed in less than or equal to 0.1 second.

9. The method, as provided in claim 1, wherein said set of measurements is greater than or equal to four signal measurements.

10. The method, as provided in claim 1, wherein said set of signal measurements is selected by use of the algorithm:

##EQU2##

11. The method, as provided in claim 1, wherein said signal measurements are performed while the test sample is flowing through said optical cell.

12. The method, as provided in claim 1, wherein said test sample consists of blood.

13. The method, as provided in claim 12, wherein said blood is hemolyzed.

14. The method, as provided in claim 1, wherein said signal measurements are performed while the test sample is stationery in said optical cell.

15. A method of performing optical flow cell transmittance measurements of inhomogeneous blood samples comprising:

(a) providing a fluid test sample to an optical cell;

(b) performing a plurality of transmittance measurements on said test sample;

(c) selecting from said transmittance measurements one or more sets of transmittance measurements having both low variability relative to the range or group of signal measurements or to defined threshold; and

either transmittance maxima;

or transmittance minima signal levels; and

(d) utilizing selected set of transmittance measurements to analyze said test sample.

16. The method of claim 15, wherein the average value of the transmittance measurements of one selected set is utilized to analyze said sample.

17. The method, as provided in claim 15, wherein said signal measurements are performed while the test sample is flowing through said optical cell.

18. The method, as provided in claim 15, wherein said signal measurements are selected by either using a temporal scan or a spatial scan of the test sample.

19. The method, as provided in claim 15, wherein said signal measurements are performed while the test sample is stationery in said optical cell.

20. In a method of performing a series of spectral signal measurements of a test sample, wherein the improvement comprises:

selecting a set of spectral measurements having both low variability, relative to the range or group of sample measurements or to a defined threshold, and one of high or low spectral signal levels, wherein said set of measurements is made at a single spectral region and is utilized for analyzing said test sample.

21. The method of claim 20, wherein the selection of sets of high or low spectral measurements is determined to avoid the effect of an inhomogeneity in the test sample to the spectral signals.

22. The method of claim 20, wherein the mean value of said signal measurements of a single set of spectral measurements is utilized to analyze said sample.

23. The method of claim 20, wherein said method further comprises:

flowing said test sample through an optical flow cell, and wherein sample measurements are taken while the test sample flows through optical flow cell.

24. The method of claim 20, wherein the variability of said measurements is determined by the formula ##EQU3##

25. The method, as provided in claim 20, wherein said signal measurements are selected by either using a temporal scan or a spatial scan of the test sample.

26. In a method of performing a plurality of spectral measurements on a test sample, wherein the improvement comprises:

selecting a set of signal measurements having low variability relative to the range or group of signal measurements or to a defined threshold; and

utilizing said selected set of measurements to analyze said test sample.

27. In a method as recited in claim 26, wherein said set of signal measurements also consists of either high or low signal levels of spectral measurement to avoid the effect of inhomogeneities in said test sample.

28. A method for multiple wavelength spectroscopic analysis of hemoglobin fractions, wherein the improvement comprises:

selecting a wavelength for an absorbance maxima of a blood sample and utilizing the results of a plurality of signal measurements at said wavelength to select a set of signal measurements from which data is provided at other wavelengths for use in determining the concentration of said hemoglobin fractions, wherein the selected set of signal measurements exhibits low variability to inhomogeneities in the blood sample.