Molecular detection apparatus and method using optical waveguide detection

• US 5,841,914 A
• Filed: 05/22/1997
• Issued: 11/24/1998
• Est. Priority Date: 04/17/1996
• Status: Expired due to Fees

• Current Assignee: Amersham Biosciences (General Electric Company)
• Original Assignee: Motorola, Inc. (Motorola Solutions, Inc.)
• Inventors: Maracas, George N., Shieh, Chan-Long, Ackley, Donald E., Harvey, Thomas B. III
• Primary Examiner(s): Healy, Brian
• Application Number:
  US08/861,927
• Time in Patent Office: 551 Days
• Field of Search: 385/12, 385/129, 385/130, 385/131, 385/132, 385/14, 385/37, 385/147, 356/128, 356/136, 356/328, 356/345, 356/351
• US Class Current: 385/12
• CPC Class Codes: G01N 21/6454 using an integrated detector array
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G02B 6/10 of the optical waveguide type G02B6/02, G02B6/24 take precedence; devices or arrangements for the control of light by electric, magnetic, electro-magnetic or acoustic means G02F1/00; transferring the modulation of modulated light G02F2/00; optical logic elements G02F3/00; optical analogue/digital converters G02F7/00

Abstract

A target molecule at a binding site is detected using an optical waveguide having a surface proximate to the binding site, and a waveguide detector coupled to the optical waveguide. An incident light beam is applied to the binding site along an axis transverse to the surface of the optical waveguide. The incident light beam impinges an optical indicator associated with the target molecule to form secondary light which is coupled into the optical waveguide. The secondary light is detected by the waveguide detector to thereby detect the target molecule.

Claims

1. A molecular detection apparatus comprising:

a substrate;

a first layer disposed on the substrate;

a photodetector disposed on the first layer, the photodetector electrically coupled to the first layer;

a second layer disposed on the photodetector, the second layer electronically coupled to the photodetector; and

an optical waveguide disposed adjacent the photodetector on the substrate, the optical waveguide having a surface defining a detection site.

2. The molecular detection apparatus of claim 1 wherein the photodetector includes a PIN diode, wherein the first layer provides an n-contact to the PIN diode, and wherein the second layer provides a p-contact to the PIN diode.

3. The molecular detection apparatus of claim 1 wherein the first layer and the second layer are formed of an ohmic material.

4. The molecular detection apparatus of claim 1 wherein the first layer and the second layer are formed of a metal.

5. The molecular detection apparatus of claim 1 wherein the first layer and the second layer provide a signal associated with a binding event at the detection site.

6. The molecular detection apparatus of claim 1 further comprising a molecular receptor at the detection site.

7. A molecular detection apparatus comprising:

an optical waveguide having a surface proximate to a molecular detection site; and

a waveguide detector coupled to the optical waveguide, the waveguide detector shaped to substantially surround the optical waveguide at the molecular detection site.

8. The molecular detection apparatus of claim 7 further comprising a substrate which supports the optical waveguide and the waveguide detector.

9. The molecular detection apparatus of claim 7 wherein the waveguide detector has an annular shape.

10. The molecular detection apparatus of claim 7 wherein the waveguide detector has a rectangular shape.