Immunogenic compositions against helicobacter infection, polypeptides for use in the compositions, and nucleic acid sequences encoding said polypeptides

• US 5,843,460 A
• Filed: 06/06/1995
• Issued: 12/01/1998
• Est. Priority Date: 05/19/1993
• Status: Expired due to Term

• Current Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH), Institut Pasteur
• Original Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH), Institut Pasteur
• Inventors: Labigne, Agnes, Suerbaum, Sebastin, Thiberge, Jean-Michel, Ferrero, Richard L.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): PORTNER, VIRGINIA ALLEN
• Application Number:
  US08/467,822
• Time in Patent Office: 1,274 Days
• Field of Search: 435/7.32, 435/4, 435/6, 435/7.9, 514/234.5, 514/41, 424/234.1
• US Class Current: 424/234.1
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/105 Delta proteobacteriales, e.g. Lawsonia; Epsilon proteobacteriales, e.g. campylobacter, helicobacter
• CPC Class Codes: C07K 14/205 from Campylobacter (G)
• CPC Class Codes: C07K 16/121 from Helicobacter (Campylobacter) (G)
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 9/80 acting on amide bonds in linear amides (3.5.1)
• CPC Class Codes: C12Q 1/689 for bacteria

Abstract

There is provided an immunogenic composition capable of inducing protective antibodies against Helicobacter infection characterized in that it comprises:

i) at least one sub-unit of a urease structural polypeptide from Helicobacter pylori (SEQ ID NOS:22,26), or a fragment thereof, said fragment being recognized by antibodies reacting with Helicobacter felis urease (SEQ ID NOS:20-21), and/or at least one sub-unit of a urease structural polypeptide from Helicobacter felis (SEQ ID NOS:20-21), or a fragment thereof, said fragment being recognized by antibodies reacting with Helicobacter pylori urease (SEQ ID NOS:22-26);

ii) and/or, a heat shock protein (Hsp), or chaperonin, from Helicobacter, or a fragment of said protein.

The preparation, by recombinant means, of such immunogenic compositions is also provided.

Claims

1. An immunogenic composition, capable of inducing antibodies against Helicobacter infection, comprising:

i) at least one urease structural polypeptide encoded by the UreB gene of Helicobacter pylori or Helicobacter felis or immunogenic fragment thereof comprising at least six consecutive amino acids; and

ii) at least one heat shock protein encoded by the Hsp A gene of Helicobacter pylori or Helicobacter felis or immunogenic fragment thereof, comprising at least 6 consecutive amino acids,

said composition being substantially free of other Helicobacter pylori or Helicobacter felis proteins.

3. The immunogenic composition according to claim 1 or claim 2, wherein the HspA is encoded by the HspA gene of plasmid pILL689 (CNCM I-1356).

4. The immunogenic composition according to claim 1 or claim 2, wherein the HspA comprises the amino acid sequence of SEQ ID NO:

1 or an immunogenic fragment thereof having at least 6 consecutive amino acids.

5. The immunogenic composition according to claim 1 or claim 2, additionally comprising an adjuvant.

6. The immunogenic composition according to claim 1 or claim 2, wherein said composition produces an immunogenic effect when administered to a mammal, wherein the immunogenic effect is substantially the same as the immunogenic effect produced in the mammal when a total cell extract of Helicobacter pylori or Helicobacter felis is administered to said mammal.

7. A method of inducing an immune response in an animal, comprising the step of administering to the animal an immunizing amount of a composition according to claim 1 or claim 2.

8. The method according to claim 7, wherein the animal is a cat or a dog.

9. The method according to claim 7, wherein the animal is a human.

10. A pharmaceutical composition for use in a vaccine against Helicobacter pylori or Helicobacter felis, comprising the immunogenic composition according to claim 1 or claim 2, in combination with a pharmaceutically acceptable carrier.

2. An immunogenic composition comprising an immunizing amount of a mixture of Helicobacter pylori or Helicobacter felis antigens, wherein said mixture consists essentially of UreB and HspA of H. pylori or H. felis substantially free of other H. pylori or H felis proteins.