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Method for detecting a target molecule in a sample using a nucleic acid ligand

  • US 5,843,653 A
  • Filed: 06/06/1995
  • Issued: 12/01/1998
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method for detecting the presence or absence of a target molecule in a sample which may contain said target molecule comprising:

  • a) exposing said sample which may contain said target molecule to a non-naturally occurring nucleic acid ligand of said target molecule, wherein said nucleic acid ligand is not a nucleic acid having the known physiological function of being bound by said target molecule, under conditions wherein a target molecule;

    nucleic acid ligand binding pair is formed if said target molecule is present, wherein said binding is not due to Watson/Crick base pairing; and

    b) determining whether said target molecule;

    nucleic acid ligand binding pair is formed, thereby detecting the presence or absence of the target molecule in the sample.

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