Methods for identification of modulatory compounds for the expression of the NES1 protein
First Claim
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1. A method of identifying a modulatory compound which is capable of increasing the expression of a Normal Epithelial Specific 1 (NES1) gene, said NES1 gene encoding a polypeptide having the sequence set forth in FIG. 10 (SEQ ID NO:
- 1), said method comprising (a) providing a cell expressing said NES1 gene, and (b) contacting said cell with a candidate compound, an increase in said NES1 expression following contact with said candidate compound identifying a modulatory compound.
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Abstract
The expression and purification of normal epithelial specific polypeptide (NES 1) which is expressed in normal cells, but not in radiation transformed cells, are described. Both the DNA sequence encoding the NES1 and the corresponding amino acid sequence are disclosed. Also, disclosed are methods for carcinoma detection and treatment using the NES1 as well as methods of identifying compounds modulating the expression and activity of NES1.
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Citations
5 Claims
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1. A method of identifying a modulatory compound which is capable of increasing the expression of a Normal Epithelial Specific 1 (NES1) gene, said NES1 gene encoding a polypeptide having the sequence set forth in FIG. 10 (SEQ ID NO:
- 1), said method comprising (a) providing a cell expressing said NES1 gene, and (b) contacting said cell with a candidate compound, an increase in said NES1 expression following contact with said candidate compound identifying a modulatory compound.
- View Dependent Claims (3, 4, 5)
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2. A method of identifying a modulatory compound which is capable of increasing NES1 protease activity, wherein said NES1 protease has the sequence set forth in FIG. 10 (SEQ ID NO:
- 1), said method comprising (a) providing a cell expressing said NES1 protease; and
(b) contacting said cell with a candidate compound, an increase in said NES1 protease activity following contact with said candidate compound identifying a modulatory compound.
- 1), said method comprising (a) providing a cell expressing said NES1 protease; and
Specification
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Current AssigneeNew England Medical Center Hospitals, Inc.
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Original AssigneeNew England Medical Center Hospitals, Inc.
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InventorsBand, Vimla
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Primary Examiner(s)Wax, Robert A.
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Assistant Examiner(s)Nashed, Nashaat T.
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Application NumberUS08/628,198Time in Patent Office970 DaysField of Search435/6, 435/7.1, 435/320.1, 435/212, 435/219, 435/23, 435/24, 435/213, 435/226, 536/23.5, 536/24.31, 530/350US Class Current435/23CPC Class CodesC12N 9/6424 Serine endopeptidases (3.4.21)
