Container for staining of cells and tissues in combination with a roller and a support

• US 5,843,793 A
• Filed: 10/10/1996
• Issued: 12/01/1998
• Est. Priority Date: 10/16/1995
• Status: Expired due to Term

• Current Assignee: Johnson & Johnson Clinical Diagnostics Inc. (Johnson & Johnson)
• Original Assignee: Johnson & Johnson Clinical Diagnostics Inc. (Johnson & Johnson)
• Inventors: Steinmann, Michele McWilliams, Chemelli, John Robert, Belly, Robert Troconis
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): GRASER, JENNIFER E
• Application Number:
  US08/728,586
• Time in Patent Office: 782 Days
• Field of Search: 436/514, 422/58, 422/68.1, 422/82.05, 422/82.09, 422/222, 422/220, 422/206, 435/287.1, 435/810, 435/287.2
• US Class Current: 436/514
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 2001/317 spraying liquids onto surfaces
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

A flexible container containing pre-deposited reagents, comprising a plurality of temporarily sealed, breakable compartments dispersed in the container, each compartment containing a reagent useful for immuno-assaying, and each compartment comprising opposed confining walls at least one of which is sufficiently flexible as to allow the compartment to be compressed in the presence of an adequate external force, external outlets in the container for the contents of said compartments, passageways extending from each of the compartments to the outlets, each of the outlets terminating in a platform that supports a drop of at least 10 μL as a pendant drop, the platforms of at least two of the outlets being adjacent so as to form between them an exterior angle with respect to each other sufficient to cause drops pendant therefrom to substantially uniformly intermix.

Claims

1. A flexible container containing predeposited reagents, comprising

a plurality of temporarily sealed, breakable compartments dispersed in the container, each compartment containing a reagent useful for immuno-assaying, and each compartment comprising opposed confining walls at least one of which is sufficiently flexible as to allow the compartment to be compressed in the presence of an adequate external force,

external outlets in the container for the contents of said compartments, said outlets being open to the atmosphere,

passageways extending from each of the compartments to the outlets, each of the outlets terminating in a platform that supports a drop of at least 10 μ

L as a pendant drop,

the platforms of at least two of the outlets being adjacent and each extending beyond their respective outlets so that the platform of one outlet contacts the platform of another of said at least two outlets so as to form between them an exterior angle with respect to each other sufficient to cause drops pendant therefrom to substantially uniformly intermix while supported by said contacting platforms.

2. A container as defined in claim 1, wherein said platforms form an exterior angle between them that is between about 180°

and about 330°

.

3. A container as defined in claim 1, wherein said exterior angle is greater than 180°

.

4. A container as defined in claim 1, wherein said platforms of said at least two outlets occupy the first and second positions of said dispersion in said container, measured from a first exterior edge of said container.

5. A container as defined in claim 1, wherein all of said compartments and passageways are free of any sample being tested.

6. A container as defined in claim 1, wherein said at least one wall will flex in the presence of an external pressure of at least 34 kPa (5 psi).

7. A container as defined in claim 1, and further including in combination therewith, a roller and means mounting said roller to cause it to compress said compartments of said container selectively, and a support to receive the contents of said compartments as they are ejected out of the container at said outlets.

8. A container as defined in claim 1, wherein one of said compartments contains a protein block solution and another of said compartments contains a solution of an antibody specific to a surface antigen of biological cells.

9. A container as defined in claim 1, wherein one of said compartments contains a solution of an antibody specific to an antigen of biological cells and another of said compartments contains a link solution.

10. A container as defined in claim 1, wherein said contacting platforms are integrally attached to each other.