Sensor utilizing living muscle cells

• US 5,846,188 A
• Filed: 01/17/1997
• Issued: 12/08/1998
• Est. Priority Date: 01/17/1997
• Status: Expired due to Term

• Current Assignee: Emissions Control Corp.
• Original Assignee: Yoram Palti
• Inventors: Palti, Yoram
• Primary Examiner(s): Lacyk, John P.
• Assistant Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US08/783,946
• Time in Patent Office: 690 Days
• Field of Search: 600/300, 600/309, 600/473, 600/476, 604/31, 604/50, 604/66, 604/67, 250/461.2
• US Class Current: 600/300
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/4519 Muscles A61B5/389, A61B5/224 take precedence

Abstract

A system and method for monitoring a constituent or a condition in a medium. Living muscle cells are immersed in the medium. The muscle cells are sensitive to the constituent or condition and respond to exposure to the constituent or condition by physical movement. An optical measuring device measures the physical movement of the muscle cells and correlates the physical movement to a level of or a change in the level of the constituent or condition in the medium. The medium may be human tissue, with the muscle cells subcutaneously implanted therein. In one embodiment, a light source transmits a light beam toward the muscle cells. A reflector mounted to the muscle cells reflects the light beam, with the reflection angle varying with the physical movement of the muscle cells. A detector detects a change in the reflection angle and a processor correlates the change in the reflection angle to a change in the level of the constituent or condition in the medium. In an alternative embodiment, a photodetector detects the amount of light transmitted through and/or around the muscle cells. Electrodes may be attached to the muscle cells for stimulating the physical movement of the muscle cells. The muscle cells may be enclosed within a capsule. The capsule includes a portion that is transparent to light so as to enable movement of the cells to be monitored.

Claims

1. A system for monitoring a constituent or a condition in a medium, the system comprising:

(a) living muscle cells which have been excised and isolated from an animal body prior to incorporation in the system, the contractile characteristics of the cells changing upon exposure to the constituent or condition in the medium with resulting physical movement of the cells; and

(b) means in optical alignment with the muscle cells for optically measuring the physical movement of the cells and for correlating the physical movement to a level of or a change in the level of the constituent or condition in the medium.

2. The system of claim 1 wherein the medium is a human body and wherein the muscle cells are adapted to be subcutaneously implanted in the body.

3. The system of claim 2 wherein the means for optically measuring the physical movement of the muscle cells is at least partially disposed outside of the human body.

4. The system of claim 3 wherein the means for optically measuring the physical movement of the muscle cells comprises a subcutaneously implanted transmitter and a receiver located outside of the body, the transmitter transmitting information related to the physical movement of the muscle cells to the receiver.

5. The system of claim 1 further comprising an output device connected to the means for optically measuring the physical movement of the muscle cells, the output device being selected from the group consisting of i) an alarm for generating an alarm signal when the level of the constituent or condition in the medium is outside of a predetermined range;

ii) a memory for storing the level of the constituent or condition over a period of time; and

iii) a display for displaying the level of the constituent or condition.

6. The system of claim 1 wherein the means for optically measuring the physical movement of the muscle cells comprises;

a light source for transmitting a light beam toward the muscle cells;

a reflector mounted to the muscle cells for reflecting the light beam, the angle of the reflection varying with the physical movement of the muscle cells; and

a detector for detecting a change in the angle of reflection.

7. The system of claim 6 further comprising:

a processor for correlating the change in the angle of reflection to a change in the level of the constituent or condition in the medium.

8. The system of claim 7 further comprising an artificial capsule which is not part of an animal body, wherein the muscle cells and the reflector are enclosed within the capsule.

9. The system of claim 8 wherein the capsule comprises i) a membrane which is impermeable to large molecules and permeable to nutrients, and ii) a portion that is transparent to light.

10. The system of claim 1 wherein the means for optically measuring the physical movement of the muscle cells comprises a light source for transmitting a light beam toward the muscle cells, and a photodetector for detecting the amount of light passing through or transmitted beyond the muscle cells.

11. The system of claim 10 wherein the muscle cells are disposed between the light source and the photodetector.

12. The system of claim 11 further comprising an artificial capsule which is not part of an animal body, wherein the muscle cells and the photodetector are enclosed within the capsule.

13. The system of claim 10 wherein the means for optically measuring the physical movement of the muscle cells further comprises a reflector, the muscle cells being disposed between the light source and the reflector, the photodetector being positioned for receiving the light reflected by the reflector.

14. The system of claim 13 further comprising an artificial capsule which is not part of an animal body, wherein the muscle cells and the reflector are enclosed within the capsule.

15. The system of claim 10 wherein the means for optically measuring the physical movement of the muscle cells further comprises a processor for correlating a change in the fraction of light passing beyond the muscle cells to the level of the constituent or condition in the medium.

16. The system of claim 1 wherein the means for optically measuring the physical movement of the muscle cells further comprises a light source for transmitting a light beam toward the muscle cells, and a photodetector for detecting the amount of light passing through the muscle cells.

17. The system of claim 1 further comprising electrodes attached to the muscle cells for stimulating the physical movement of the muscle cells.

18. A method of monitoring a constituent or a condition in a medium, the method comprising the steps of:

(a) providing living muscle cells which have been excised and isolated from an animal body prior to use in the method, the contractile characteristics of the cells changing upon exposure to the constituent or condition in the medium with resulting physical movement of the cells, and placing the living cells in the medium;

(b) optically measuring the physical movement of the muscle cells; and

(c) correlating the physical movement of the muscle cells to a level of or a change in the level of the constituent or condition in the medium.

19. The method of claim 18 wherein the medium is a human body and further comprising the step of surgically or hypodermically subcutaneously implanting the muscle cells.

20. The method of claim 18 wherein the optical measuring step comprises the steps of i) attaching a reflector to the muscle cells, ii) transmitting a beam of light at the reflector, the light beam being reflected at an angle by the reflector, the angle being related to the physical movement of the muscle cells, and iii) detecting the change in the angle of reflection.

21. The method of claim 20 wherein the muscle cells and the reflector are enclosed within an artificial capsule which is not part of an animal body.

22. The method of claim 18 wherein the correlating step comprises comparing the physical movement of the muscle cells to a predetermined response characteristic for the muscle cells, the response characteristic relating physical movement of the muscle cells responsive to the constituent or condition to the level of or a change in the level of the constituent or condition.

23. The method of claim 18 wherein the optical measuring step comprises transmitting a light beam toward the muscle cells, and detecting the amount of light passing through or transmitted beyond the muscle cells.

24. The method of claim 18 wherein the correlating step comprises correlating a change in the amount of light passing through or transmitted beyond the muscle cells to the level of the constituent or condition in the medium.

25. The method of claim 18 further comprising the step of stimulating the muscle cells prior to the optical measuring step.

26. A system for monitoring a constituent or a condition in a medium, the system comprising:

(a) solid, living muscle cells which have been excised and isolated from an animal body prior to incorporation in the system, the contractile characteristics of the cells changing upon exposure to the constituent or condition in the medium with resulting physical movement;

(b) an artificial capsule surrounding the living muscle cells, the capsule not being part of an animal body and comprising a transparent portion; and

(c) means for optically measuring the physical movement of the muscle cells through the transparent portion of the capsule and for correlating the physical movement to a level of or a change in the level of the constituent or condition in the medium.